Development of a stable gaussia luciferase enterovirus 71 reporter virus
文献类型：期刊论文
| 作者 | Xu, Lin-Lin1; Shan, Chao1; Deng, Cheng-Lin1,2; Li, Xiao-Dan1,2; Shang, Bao-Di3; Ye, Han-Qing1; Liu, Si-Qing1; Yuan, Zhi-Ming2; Wang, Qing-Yin4; Shi, Pei-Yong4 |
| 刊名 | Journal of virological methods
 |
| 出版日期 | 2015-07-01 |
| 卷号 | 219页码:62-66 |
| ISSN号 | 0166-0934 |
| 关键词 | Enterovirus Reporter virus Gaussia luciferase Antiviral Replication |
| DOI | 10.1016/j.jviromet.2015.03.020 |
| 通讯作者 | Zhang, bo(zhangbo@wh.iov.cn) |
| 英文摘要 | We report a stable gaussia luciferase enterovirus 71 (gluc-ev71) reporter virus to facilitate drug discovery. the gluc-ev71 reporter virus was generated by engineering the gaussia luciferase (gluc) gene between the 5' untranslated region and vp4 gene of the ev71 genome. we could recover gluc-ev71 after transfection of vero cells with the cdna clone-derived rna. the reporter virus efficiently infects and replicates in various cell types (vero, human rhabdomyosarcoma, and hela cells), producing robust luciferase activity. the gluc-ev71 virus replicates slower than the wild-type virus in cell culture. the reporter virus is stable in maintaining the gluc gene after five rounds of continuous passaging in vero cells. using known ev71 inhibitors, we demonstrate that the reporter virus can be used for antiviral testing. however, the gluc-ev71 infection assay cannot be adapted to a homogenous format for high throughput screen, mainly due to the secreted nature of the gluc protein and the short half-life of the gluc luminescence signal. the gluc-ev71 and its infection assay could be useful for antiviral drug discovery as well as for studying ev71 replication and pathogenesis. (c) 2015 elsevier b.v. all rights reserved. |
| WOS关键词 | REPLICATION ; POLIOVIRUS ; IDENTIFICATION ; MUTATIONS ; RECEPTOR ; CELLS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000355040500010 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375174 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Bo |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430071, Peoples R China 3.Guizhou Fisheries Res Inst, Guiyang 550025, Peoples R China 4.Novartis Inst Trop Dis, Singapore 138670, Singapore |
| 推荐引用方式 GB/T 7714 | Xu, Lin-Lin,Shan, Chao,Deng, Cheng-Lin,et al. Development of a stable gaussia luciferase enterovirus 71 reporter virus[J]. Journal of virological methods,2015,219:62-66. |
| APA | Xu, Lin-Lin.,Shan, Chao.,Deng, Cheng-Lin.,Li, Xiao-Dan.,Shang, Bao-Di.,...&Zhang, Bo.(2015).Development of a stable gaussia luciferase enterovirus 71 reporter virus.Journal of virological methods,219,62-66. |
| MLA | Xu, Lin-Lin,et al."Development of a stable gaussia luciferase enterovirus 71 reporter virus".Journal of virological methods 219(2015):62-66. |
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来源：武汉病毒研究所
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