The application of alkaline phosphatase labeled hbv probe in serum detection
文献类型：期刊论文
| 作者 | Tu, ZM; Ke, LH; He, GY |
| 刊名 | Virus genes
 |
| 出版日期 | 2004-03-01 |
| 卷号 | 28期号:2页码:151-156 |
| 关键词 | Alkaline phosphatase Cross-linking Hbv probe Hepatitis Non-radioactive Serum detection Virus dna |
| ISSN号 | 0920-8569 |
| 通讯作者 | Tu, zm(tuzhiming9@sina.com) |
| 英文摘要 | A method using non-radioactive material alkaline phosphatase to label hbv dna as probe has been studied and used in clinical experiments to detect the hbv dna in hepatitis serum. alkaline phosphatase coupled with polyethyleneimine (pei) using p-benzoquine as cross-linking reagent. the modified phosphatase was covalently linked to single strand dna using glutraldehyde. such single strand dna enzyme complexes have been tested for blot hybridization, after hybridization and incubation with a substrate solution, sequences complementary to the probe can be visualized directly in 1 h. the minimum amount about 10 pg of target dna has been detected in this way, 32 p labeled probes are autoradiography 1 h after hybridization can only detect 10 ng, so the enzyme labeled probe is more sensitive than isotope labeled probe in 1 h fast test. comparing the enzyme-labeled hbv dna probe with 32 p labeled the same one, positive proportion of detecting the hbv dna in hepatitis patients was about 95.7%. because the positive patient's serum detected by 32 p labeled probe were selected through 1-week radiation, alkaline phosphatase labeled probes are color developed for only 1 h. our experiment certified that it is a sensitive, specific, easy, rapid, safe and economical probe labeling and clinical virus dna detection method. |
| WOS关键词 | INSITU HYBRIDIZATION ; BLOT HYBRIDIZATION ; DNA PROBES ; COLORIMETRIC METHOD ; NUCLEIC-ACIDS ; BIOTIN ; RNA ; QUANTITIES ; SEQUENCES ; ASSAY |
| WOS研究方向 | Genetics & Heredity ; Virology |
| WOS类目 | Genetics & Heredity ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000189075400001 |
| 出版者 | KLUWER ACADEMIC PUBL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375187 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Tu, ZM |
| 作者单位 | 1.Huazhong Univ Sci & Technol, Sch Life Sci & Technol, China UK HUST RRes Genet Engn & Gen Joint Lab, Wuhan 430074, Peoples R China 2.Acad Sinica, Wuhan Inst Virol, Wuhan 430071, Peoples R China 3.Huazhong Univ Sci & Technol, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tu, ZM,Ke, LH,He, GY. The application of alkaline phosphatase labeled hbv probe in serum detection[J]. Virus genes,2004,28(2):151-156. |
| APA | Tu, ZM,Ke, LH,&He, GY.(2004).The application of alkaline phosphatase labeled hbv probe in serum detection.Virus genes,28(2),151-156. |
| MLA | Tu, ZM,et al."The application of alkaline phosphatase labeled hbv probe in serum detection".Virus genes 28.2(2004):151-156. |
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来源：武汉病毒研究所
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