Towards a molecular identification and classification system of lepidopteran-specific baculoviruses
文献类型：期刊论文
| 作者 | Lange, M; Wang, HL; Zhihong, H; Jehle, JA |
| 刊名 | Virology
 |
| 出版日期 | 2004-07-20 |
| 卷号 | 325期号:1页码:36-47 |
| 关键词 | Baculoviridae Nucleopolyhedrovirus Granulovirus Virus identification Molecular phylogeny Polymerase chain reaction |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2004.04.023 |
| 通讯作者 | Jehle, ja() |
| 英文摘要 | Virus genomics provides novel approaches for virus identification and classification. based on the comparative analyses of sequenced lepidopteran-specific baculovirus genomes, degenerate oligonucleotides were developed that allow the specific amplification of several regions of the genome using polymerase chain reaction (pcr) followed by dna sequencing. the dna sequences within the coding regions of three highly conserved genes, namely polyhedrin/granulin (polh/gran), late expression factor 8 (lef-8), and late expression factor 9 (lef-9), were targeted for amplification. the oligonucleotides were tested on viral dnas isolated from historical field samples, and amplification products were generated from 12 isolated nucleopolyhedrovirus (npv) and 8 granulovirus (gv) dnas. the pcr products were cloned or directly sequenced, and phylogenetic trees were inferred from individual and combined data sets of these three genes and compared to a phylogeny, which includes 22 baculoviruses using a combined data set of 30 core genes. this method allows a fast and reliable detection and identification of lepidopteran-specific npvs and gvs. furthermore, a strong correlation of the base composition of these three genome areas with that of the complete virus genome was observed and used to predict the base composition of uncharacterized baculovirus genomes. these analyses suggested that gvs have a significantly higher at content than npvs. (c) 2004 elsevier inc. all rights reserved. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; SEQUENCE-ANALYSIS ; GENOME SEQUENCE ; NUCLEOPOLYHEDROVIRUS GENOME ; GRANULOVIRUS GENOME ; MAMESTRA-CONFIGURATA ; CULEX-NIGRIPALPUS ; RACHIPLUSIA-OU ; ORGANIZATION ; GENE |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000222640400005 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375194 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Jehle, JA |
| 作者单位 | 1.Agr Serv Ctr Palatinate, Lab Biotechnol Crop Protect, Dept Phytopathol, D-67435 Neustadt, Germany 2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Mol Virol, Wuhan, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Pathol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lange, M,Wang, HL,Zhihong, H,et al. Towards a molecular identification and classification system of lepidopteran-specific baculoviruses[J]. Virology,2004,325(1):36-47. |
| APA | Lange, M,Wang, HL,Zhihong, H,&Jehle, JA.(2004).Towards a molecular identification and classification system of lepidopteran-specific baculoviruses.Virology,325(1),36-47. |
| MLA | Lange, M,et al."Towards a molecular identification and classification system of lepidopteran-specific baculoviruses".Virology 325.1(2004):36-47. |
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来源：武汉病毒研究所
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