Heterologous expression of alkene monooxygenase components from xanthobacter autotrophicus py2 and reconstitution of the active complex
文献类型：期刊论文
| 作者 | Champreda, V; Zhou, NY; Leak, DJ |
| 刊名 | Fems microbiology letters
 |
| 出版日期 | 2004-10-15 |
| 卷号 | 239期号:2页码:309-318 |
| 关键词 | Monooxygenase Xanthobacter Component interaction Coupling protein Ferredoxin |
| ISSN号 | 0378-1097 |
| DOI | 10.1016/j.femsle.2004.09.002 |
| 通讯作者 | Leak, dj() |
| 英文摘要 | The coupling protein and ferredoxin from xanthobacter autotrophicus py2 alkene monooxygenase (xamo) have been functionally expressed in both n-terminal affinity tagged fusion and native forms in escherichia coli. however, attempts to express the nadh-oxidoreductase and oxygenase, always resulted in the production of inactive, insoluble proteins. nevertheless, the recombinant reductase from the toluene 4-monooxygenase of pseudomonas mendocina kr1 was found to functionally complement the xamo system. in vitro reconstitution, using the recombinant coupling protein and other components purified from the wild type, showed that steady-state epoxidation rate and coupling efficiency were dependent on the relative concentration of xamo components in the reaction. the optimal molar stoichiometric ratio of xamo components was determined to be approximately 1:0.25-0.3:2:2 (oxygenase hexamer:reductase:ferredoxin:coupling protein), suggesting the formation of an efficient catalytic complex at the minimal stoichiometric ratio to saturate the probable two-fold symmetry binding sites on the oxygenase. (c) 2004 federation of european microbiological societies. published by elsevier b.v. all rights reserved. |
| WOS关键词 | SOLUBLE METHANE MONOOXYGENASE ; METHYLOCOCCUS-CAPSULATUS BATH ; METHYLOSINUS-TRICHOSPORIUM OB3B ; PROTEIN-B ; TOLUENE 4-MONOOXYGENASE ; REGULATORY PROTEIN ; ALIPHATIC ALKENES ; SEQUENCE-ANALYSIS ; ESCHERICHIA-COLI ; STRAIN PY2 |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000224680200015 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375199 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Leak, DJ |
| 作者单位 | 1.Univ London Imperial Coll Sci Technol & Med, Dept Biol Sci, London SW7 2AZ, England 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Champreda, V,Zhou, NY,Leak, DJ. Heterologous expression of alkene monooxygenase components from xanthobacter autotrophicus py2 and reconstitution of the active complex[J]. Fems microbiology letters,2004,239(2):309-318. |
| APA | Champreda, V,Zhou, NY,&Leak, DJ.(2004).Heterologous expression of alkene monooxygenase components from xanthobacter autotrophicus py2 and reconstitution of the active complex.Fems microbiology letters,239(2),309-318. |
| MLA | Champreda, V,et al."Heterologous expression of alkene monooxygenase components from xanthobacter autotrophicus py2 and reconstitution of the active complex".Fems microbiology letters 239.2(2004):309-318. |
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来源：武汉病毒研究所
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