Identification of two critical amino acid residues of the severe acute respiratory syndrome coronavirus spike protein for its variation in zoonotic tropism transition via a double substitution strategy
文献类型：期刊论文
| 作者 | Qu, XX; Hao, P; Song, XJ; Jiang, SM; Liu, YX; Wang, PG; Rao, X; Song, HD; Wang, SY; Zuo, Y |
| 刊名 | Journal of biological chemistry
 |
| 出版日期 | 2005-08-19 |
| 卷号 | 280期号:33页码:29588-29595 |
| ISSN号 | 0021-9258 |
| DOI | 10.1074/jbc.m500662200 |
| 通讯作者 | Zhao, gp() |
| 英文摘要 | Severe acute respiratory syndrome coronavirus (sars-cov) is a recently identified human coronavirus. the extremely high homology of the viral genomic sequences between the viruses isolated from human (husars-cov) and those of palm civet origin (pcsarscov) suggested possible palm civet-to-human transmission. genetic analysis revealed that the spike ( s) protein of pcsars-cov and husars-cov was subjected to the strongest positive selection pressure during transmission, and there were six amino acid residues within the receptor-binding domain of the s protein being potentially important for sars progression and tropism. using the single-round infection assay, we found that a two-amino acid substitution (n479k/t487s) of a husars-cov for those of pcsarscov almost abolished its infection of human cells expressing the sars-cov receptor ace2 but no effect upon the infection of mouse ace2 cells. although single substitution of these two residues had no effects on the infectivity of husars-cov, these recombinant s proteins bound to human ace2 with different levels of reduced affinity, and the two-amino acid-substituted s protein showed extremely low affinity. on the contrary, substitution of these two amino acid residues of pcsars-cov for those of husras-cov made pcsars-cov capable of infecting human ace2-expressing cells. these results suggest that amino acid residues at position 479 and 487 of the s protein are important determinants for sars-cov tropism and animal-to-human transmission. |
| WOS关键词 | ANGIOTENSIN-CONVERTING ENZYME-2 ; SARS-CORONAVIRUS ; AMINOPEPTIDASE-N ; FUNCTIONAL-CHARACTERIZATION ; NEUTRALIZING ANTIBODIES ; EFFICIENT REPLICATION ; VIRUS TYPE-1 ; RECEPTOR ; GLYCOPROTEIN ; EXPRESSION |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000231176200026 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375200 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhao, GP |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Ctr Hlth Sci, Bioinformat Ctr,Inst Plant Physiol & Ecol, Shanghai 200031, Peoples R China 2.Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China 3.Shanghai Med Univ 2, Ruijin Hosp, Pole Sinfrancais Rech Sci Vivant & Genom, State Key Lab Med Genom, Shanghai 200025, Peoples R China 4.Chinese Natl Human Genome Ctr, Shanghai 201203, Peoples R China 5.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 6.Fudan Univ, Sch Life Sci, Dept Microbiol, State Key lab Genet Engn, Shanghai 200433, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qu, XX,Hao, P,Song, XJ,et al. Identification of two critical amino acid residues of the severe acute respiratory syndrome coronavirus spike protein for its variation in zoonotic tropism transition via a double substitution strategy[J]. Journal of biological chemistry,2005,280(33):29588-29595. |
| APA | Qu, XX.,Hao, P.,Song, XJ.,Jiang, SM.,Liu, YX.,...&Deng, HK.(2005).Identification of two critical amino acid residues of the severe acute respiratory syndrome coronavirus spike protein for its variation in zoonotic tropism transition via a double substitution strategy.Journal of biological chemistry,280(33),29588-29595. |
| MLA | Qu, XX,et al."Identification of two critical amino acid residues of the severe acute respiratory syndrome coronavirus spike protein for its variation in zoonotic tropism transition via a double substitution strategy".Journal of biological chemistry 280.33(2005):29588-29595. |
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来源：武汉病毒研究所
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