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Molecular characterization of a major outer capsid protein encoded by the threadfin aquareovirus (tfv) gene segment 10 (s10)

文献类型:期刊论文

作者Seng, EK; Fang, Q; Sin, YM; Lam, TJ
刊名Archives of virology
出版日期2005-10-01
卷号150期号:10页码:2021-2036
ISSN号0304-8608
DOI10.1007/s00705-005-0550-9
通讯作者Seng, ek(dbssek@nus.edu.sg)
英文摘要Genome segment 10 (s10) of threadfin aquareovirus (tfv) was cloned, sequenced, analyzed and found to be 987 bp long encoding a protein of 298 aa with a predicted molecular mass of 32.0 kda. the tfv s10 gene possesses terminal motifs, (5' gtttta and attcatc 3') which are also conserved in the s6 and s11 tfv gene segments. sequence comparison revealed that the tfv s10 gene was similar to the striped bass reovirus (sbr) vp7 outer capsid protein (ocp). a conserved putative zinc-finger motif, cchc, present in the mammalian reovirus (mrv) delta 3 protein, was identified in tfv and other aquareovirus vp7 protein. phylogenetic analysis of the tfv vp7 protein indicated that tfv is closely related to sbr and chum salmon reovirus (csv) and possibly belong to the same species aquareovirus a as sbr and csv. the tfv vp7 protein was expressed in e. coli, purified and injected into mice. serum specific antibodies were generated, however, the serum showed weak neutralizing activity. in contrast, co-incubation of this serum with another serum obtained from mice immunized with another ocp encoded by the tfv s6 gene segment resulted in a highly elevated antibody neutralization titer.
WOS关键词RNA BLOT HYBRIDIZATION ; SEQUENCE-ANALYSIS ; ROTAVIRUS ANTIGENICITY ; REOVIRUS ; FISH ; GENOGROUP ; VIRUSES ; VP7 ; IDENTIFICATION ; GLYCOSYLATION
WOS研究方向Virology
WOS类目Virology
语种英语
WOS记录号WOS:000232030100007
出版者SPRINGER WIEN
URI标识http://www.irgrid.ac.cn/handle/1471x/2375216
专题武汉病毒研究所
通讯作者Seng, EK
作者单位1.Natl Univ Singapore, Dept Biol Sci, Virol Lab 05 02, Singapore 117543, Singapore
2.CAS, Wuhan Inst Virol, Wuhan, Peoples R China
3.Teo Way Yong & Sons Pte Ltd, Singapore Fish Breeding & Immunizat Ctr, Singapore, Singapore
推荐引用方式
GB/T 7714
Seng, EK,Fang, Q,Sin, YM,et al. Molecular characterization of a major outer capsid protein encoded by the threadfin aquareovirus (tfv) gene segment 10 (s10)[J]. Archives of virology,2005,150(10):2021-2036.
APA Seng, EK,Fang, Q,Sin, YM,&Lam, TJ.(2005).Molecular characterization of a major outer capsid protein encoded by the threadfin aquareovirus (tfv) gene segment 10 (s10).Archives of virology,150(10),2021-2036.
MLA Seng, EK,et al."Molecular characterization of a major outer capsid protein encoded by the threadfin aquareovirus (tfv) gene segment 10 (s10)".Archives of virology 150.10(2005):2021-2036.

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来源:武汉病毒研究所

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