An imaging study using laminin peptide tc-99m-yigsr in mice bearing ehrlich ascites tumour
文献类型：期刊论文
| 作者 | Hu, J; Zhang, YX; Lan, XL; Qin, GM; Zhang, J; Hu, ZH |
| 刊名 | Chinese medical journal
 |
| 出版日期 | 2005-05-05 |
| 卷号 | 118期号:9页码:753-758 |
| 关键词 | Radionuclide imaging Tc-99m-yigsr Laminin receptors |
| ISSN号 | 0366-6999 |
| 通讯作者 | Hu, j(zhoudai2004@hotmail.com) |
| 英文摘要 | Background the yigsr is a pentapeptide, from the laminin-1 of the β 1 chain, which can mediate cell adhesion and bind the 67 kd laminin receptor. the purpose is to evaluate the usefulness of tc-99m-yigsr, a novel tumour radiotracer, in the receptor imaging of ehrlich ascites tumour. methods using s-acetly-nh3-mag(3) as chelate, yigsr, a pentapeptide from laminin, was tagged with tc-99m. tc-99m-yigsr was detected in the tumour group bearing ehrlich ascites tumour and blocked group. tumour, normal, inflammatory and blocked groups were imaged. results through reverse phase sep-pak c-18 chromatogram, it was revealed that yigsr could conjugate with s-acetly-nh3-mag(3), and be radiolabelled at room temperature and neutral ph with a radiolabelling yield of 62%, and of 4% without chelate. tc-99m-yigsr was rapidly cleared from kidney, then liver. the imaging findings showed tumour tissue accumulated initial radioactivity at fifteen minutes after injection in the tumour group, and the uptake increased to peak at three hours with a tumour/muscle ratio (t/m) of 11.36, then cleared slowly to a t/m of 7.50 at eight hours. the tumour uptake of radiotracer in blocked group was significantly lower with t/m of 4.61 at three hours and 0.89 at eight hours. the t/m was only 3.72 at three hours and 1.29 at eight hours after injection in inflammatory group. compared with inflammatory group and control obstructive group, the ratio of t/m in tumour group was significantly different (p < 0.001). conclusions using s-acetly-nh3-mag(3), we radiolabelled yigsr with tc-99m. tc-99m-yigsr possesses many merits of tumour imaging. rapid visualization, high sensitivity and specificity, and satisfactory target/nontarget ratio. our data suggest tc-99m-yigsr is a promising tumour radiotracer. |
| WOS关键词 | CARCINOMA-CELLS ; BINDING PROTEIN ; EXPRESSION ; RECEPTOR ; LOCALIZATION ; YIGSR ; CHAIN |
| WOS研究方向 | General & Internal Medicine |
| WOS类目 | Medicine, General & Internal |
| 语种 | 英语 |
| WOS记录号 | WOS:000229172400009 |
| 出版者 | CHINESE MEDICAL ASSOCIATION |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375219 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Hu, J |
| 作者单位 | 1.Huazhong Univ Sci & Technol, Union Hosp, Dept Nucl Med, Wuhan 430022, Peoples R China 2.Shenzhen Second Peoples Hosp, Dept Nucl Med, Shenzhen 518035, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, J,Zhang, YX,Lan, XL,et al. An imaging study using laminin peptide tc-99m-yigsr in mice bearing ehrlich ascites tumour[J]. Chinese medical journal,2005,118(9):753-758. |
| APA | Hu, J,Zhang, YX,Lan, XL,Qin, GM,Zhang, J,&Hu, ZH.(2005).An imaging study using laminin peptide tc-99m-yigsr in mice bearing ehrlich ascites tumour.Chinese medical journal,118(9),753-758. |
| MLA | Hu, J,et al."An imaging study using laminin peptide tc-99m-yigsr in mice bearing ehrlich ascites tumour".Chinese medical journal 118.9(2005):753-758. |
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来源：武汉病毒研究所
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