Identification of cry-type genes on 20-kb dna associated with cry1 crystal proteins from bacillus thuringiensis
文献类型：期刊论文
| 作者 | Xia, LQ; Sun, YJ; Ding, XZ; Fu, ZJ; Mo, XT; Zhang, H; Yuan, ZM |
| 刊名 | Current microbiology
 |
| 出版日期 | 2005-07-01 |
| 卷号 | 51期号:1页码:53-58 |
| ISSN号 | 0343-8651 |
| DOI | 10.1007/s00284-005-4504-y |
| 通讯作者 | Xia, lq(xialq@hunnu.edu.cn) |
| 英文摘要 | Heterologous dna fragments (20-kb) associated with cry i crystal proteins (protoxins) from a soil-isolated bacillus thuringiensis strain 4.0718 were isolated and analyzed. rflp patterns of the pcr products showed that the 20-kb dna fragments harbored cry1aa, cry1ac, cry2aa, and cry2ab genes. furthermore, a 4.2-kb dna fragment, which contained the promoter, the coding region, and the terminator of cry1ac gene, was cloned from the 20-kb dnas by pcr, and then the cry1ac gene was expressed in an acrystalliferous b. thuringiensis strain 4q7 by using e. coli-b. thuringiensis shuttle vector pht3101. sds-page and microscopy studies revealed that the recombinant could express 130-kda cry1ac protoxin and produce bipyramidal crystals during sporulation. bioassay results proved that crystal-spore mixture from the recombinant was toxic to plutella xylostella. this was the first report of cry-type genes present on 20-kb dna associated with cry1 protoxins of b. thuringiensis. |
| WOS关键词 | AMINO-ACID-SEQUENCE ; DELTA-ENDOTOXIN GENE ; NUCLEOTIDE-SEQUENCE ; KURSTAKI ; PROTOXIN ; CLONING ; LENGTH ; TOXIN |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000230818800011 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375225 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Xia, LQ |
| 作者单位 | 1.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, LQ,Sun, YJ,Ding, XZ,et al. Identification of cry-type genes on 20-kb dna associated with cry1 crystal proteins from bacillus thuringiensis[J]. Current microbiology,2005,51(1):53-58. |
| APA | Xia, LQ.,Sun, YJ.,Ding, XZ.,Fu, ZJ.,Mo, XT.,...&Yuan, ZM.(2005).Identification of cry-type genes on 20-kb dna associated with cry1 crystal proteins from bacillus thuringiensis.Current microbiology,51(1),53-58. |
| MLA | Xia, LQ,et al."Identification of cry-type genes on 20-kb dna associated with cry1 crystal proteins from bacillus thuringiensis".Current microbiology 51.1(2005):53-58. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。