Induction of th1 type response by dna vaccinations with n, m, and e genes against sars-cov in mice
文献类型：期刊论文
| 作者 | Jin, HL; Xiao, C; Chen, Z; Kang, YM; Ma, YJ; Zhu, KC; Xie, QF; Tu, YX; Yu, Y; Wang, B |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2005-03-25 |
| 卷号 | 328期号:4页码:979-986 |
| 关键词 | Sars-cov Nucleocapsid protein Membrane protein Envelop protein Dna vaccine In vivo ctl Th1 response |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2005.01.048 |
| 通讯作者 | Wang, b() |
| 英文摘要 | Vaccination against the sars-cov infection is an attractive means to control the spread of viruses in public. in this study, we employed a dna vaccine technology with the levamisole, out, newly discovered chemical adjuvant, to generate th1 type of response. to avoid the enhancement antibody issue, genes encoding the nucleocapsid, membrane, and envelope protein of sars-cov were cloned and their expressions in mammalian cells were determined. after the intramuscular introduction into animals, we observed that the constructs of the e, m, and n genes could induce high levels of specific antibodies, t cell proliferations, ifn-gamma, dth responses, and in vivo cytotoxic t cells activities specifically against sars-cov antigens. the highest immune responses were generated by the construct encoding the nucleocapsid protein. the results suggest that the n, m, and e genes could be used as the targets to prevent sars-cov infection in the dna vaccine development. (c) 2005 elsevier inc. all rights reserved. |
| WOS关键词 | FELINE INFECTIOUS PERITONITIS ; B SURFACE-ANTIGEN ; IMMUNE-RESPONSES ; NUCLEOCAPSID PROTEIN ; ALPHA-INTERFERON ; VIRUS CHALLENGE ; CTL EPITOPES ; PLASMID DNA ; T-CELL ; PROTECTION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000227233000024 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375227 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, B |
| 作者单位 | 1.China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 3.Xinjiang Univ, Coll Life Sci & Technol, Urumqi 830046, Xinjiang, Peoples R China 4.Xinjiang Univ, Key Lab Bioresources & Bioengn, Urumqi 830046, Xinjiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jin, HL,Xiao, C,Chen, Z,et al. Induction of th1 type response by dna vaccinations with n, m, and e genes against sars-cov in mice[J]. Biochemical and biophysical research communications,2005,328(4):979-986. |
| APA | Jin, HL.,Xiao, C.,Chen, Z.,Kang, YM.,Ma, YJ.,...&Wang, B.(2005).Induction of th1 type response by dna vaccinations with n, m, and e genes against sars-cov in mice.Biochemical and biophysical research communications,328(4),979-986. |
| MLA | Jin, HL,et al."Induction of th1 type response by dna vaccinations with n, m, and e genes against sars-cov in mice".Biochemical and biophysical research communications 328.4(2005):979-986. |
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来源：武汉病毒研究所
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