中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Isolation and characterization of phytoene desaturase cdna involved in the beta-carotene biosynthetic pathway in dunaliella salina

文献类型:期刊论文

作者Zhu, YH; Jiang, JG; Yan, Y; Chen, XW
刊名Journal of agricultural and food chemistry
出版日期2005-07-13
卷号53期号:14页码:5593-5597
关键词Dunaliella salina Beta-carotene biosynthesis Phytoene desaturase cdna Smart technology
ISSN号0021-8561
DOI10.1021/jf0506838
英文摘要The green alga dunaliella salina is one of the best and most important biological sources of beta-carotene; however, to date the molecular basis of the p-carotene biosynthesis process in d. salina is still unresolved. the dehydrogenation of phytoene is the second step in the carotenoids biosynthetic pathway, and the phytoene-related desaturases are the key enzymes in the beta-carotene biosynthetic pathway. a phytoene desaturase (pds) cdna with a 1752 bp open reading frame was cloned by rt-pcr and race-pcr methods on the basis of a modified switching mechanism at 5' end of the rna transcript (smart) technology from d. salina. the predicted protein sequence displays a high identity (up to 65%) with phytoene desaturases of higher plants and cyanobacteria. the highest amino acid sequence identity (91%) is shared with the phytoene desaturase sequence of dunaliella bardawil, and a dinucleotide-binding motif lies in the n-terminal. the phylogenetic analysis shows that d. salina pds is closer to higher plants and cyanobacteria than bacterial and fungi. these results together demonstrated the cloned pds cdna of d. salina is a pds-type gene, and it is postulated that in d. salina the first two dehydrogenations, by which phytoene is converted into zeta-carotene, are carried out by this putative phytoene desaturase.
WOS关键词ZETA-CAROTENE ; PLANTS ; GENE ; CAROTENOGENESIS ; IDENTIFICATION ; SEQUENCE ; CLONING
WOS研究方向Agriculture ; Chemistry ; Food Science & Technology
WOS类目Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
语种英语
WOS记录号WOS:000230357200018
出版者AMER CHEMICAL SOC
URI标识http://www.irgrid.ac.cn/handle/1471x/2375232
专题武汉病毒研究所
作者单位1.S China Univ Technol, Coll Food & Biotechnol, Guangzhou 510640, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China
推荐引用方式
GB/T 7714
Zhu, YH,Jiang, JG,Yan, Y,et al. Isolation and characterization of phytoene desaturase cdna involved in the beta-carotene biosynthetic pathway in dunaliella salina[J]. Journal of agricultural and food chemistry,2005,53(14):5593-5597.
APA Zhu, YH,Jiang, JG,Yan, Y,&Chen, XW.(2005).Isolation and characterization of phytoene desaturase cdna involved in the beta-carotene biosynthetic pathway in dunaliella salina.Journal of agricultural and food chemistry,53(14),5593-5597.
MLA Zhu, YH,et al."Isolation and characterization of phytoene desaturase cdna involved in the beta-carotene biosynthetic pathway in dunaliella salina".Journal of agricultural and food chemistry 53.14(2005):5593-5597.

入库方式: iSwitch采集

来源:武汉病毒研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。