Isolation and characterization of phytoene desaturase cdna involved in the beta-carotene biosynthetic pathway in dunaliella salina
文献类型：期刊论文
| 作者 | Zhu, YH; Jiang, JG; Yan, Y; Chen, XW |
| 刊名 | Journal of agricultural and food chemistry
 |
| 出版日期 | 2005-07-13 |
| 卷号 | 53期号:14页码:5593-5597 |
| 关键词 | Dunaliella salina Beta-carotene biosynthesis Phytoene desaturase cdna Smart technology |
| ISSN号 | 0021-8561 |
| DOI | 10.1021/jf0506838 |
| 英文摘要 | The green alga dunaliella salina is one of the best and most important biological sources of beta-carotene; however, to date the molecular basis of the p-carotene biosynthesis process in d. salina is still unresolved. the dehydrogenation of phytoene is the second step in the carotenoids biosynthetic pathway, and the phytoene-related desaturases are the key enzymes in the beta-carotene biosynthetic pathway. a phytoene desaturase (pds) cdna with a 1752 bp open reading frame was cloned by rt-pcr and race-pcr methods on the basis of a modified switching mechanism at 5' end of the rna transcript (smart) technology from d. salina. the predicted protein sequence displays a high identity (up to 65%) with phytoene desaturases of higher plants and cyanobacteria. the highest amino acid sequence identity (91%) is shared with the phytoene desaturase sequence of dunaliella bardawil, and a dinucleotide-binding motif lies in the n-terminal. the phylogenetic analysis shows that d. salina pds is closer to higher plants and cyanobacteria than bacterial and fungi. these results together demonstrated the cloned pds cdna of d. salina is a pds-type gene, and it is postulated that in d. salina the first two dehydrogenations, by which phytoene is converted into zeta-carotene, are carried out by this putative phytoene desaturase. |
| WOS关键词 | ZETA-CAROTENE ; PLANTS ; GENE ; CAROTENOGENESIS ; IDENTIFICATION ; SEQUENCE ; CLONING |
| WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
| WOS类目 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000230357200018 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375232 |
| 专题 | 武汉病毒研究所 |
| 作者单位 | 1.S China Univ Technol, Coll Food & Biotechnol, Guangzhou 510640, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, YH,Jiang, JG,Yan, Y,et al. Isolation and characterization of phytoene desaturase cdna involved in the beta-carotene biosynthetic pathway in dunaliella salina[J]. Journal of agricultural and food chemistry,2005,53(14):5593-5597. |
| APA | Zhu, YH,Jiang, JG,Yan, Y,&Chen, XW.(2005).Isolation and characterization of phytoene desaturase cdna involved in the beta-carotene biosynthetic pathway in dunaliella salina.Journal of agricultural and food chemistry,53(14),5593-5597. |
| MLA | Zhu, YH,et al."Isolation and characterization of phytoene desaturase cdna involved in the beta-carotene biosynthetic pathway in dunaliella salina".Journal of agricultural and food chemistry 53.14(2005):5593-5597. |
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来源：武汉病毒研究所
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