Intracellular dopamine oxidation mediates rotenone-induced apoptosis in pc12 cells
文献类型：期刊论文
| 作者 | Liu, HQ; Zhu, XZ; Weng, EQ |
| 刊名 | Acta pharmacologica sinica
 |
| 出版日期 | 2005 |
| 卷号 | 26期号:1页码:17-26 |
| 关键词 | Parkinson disease Rotenone Reserpine Deprenyl Reactive oxygen species Neurotoxicity |
| ISSN号 | 1671-4083 |
| DOI | 10.1117/j.1745-7254.2005.00003.x |
| 通讯作者 | Zhu, xz(xzzhu@mail.shcnc.ac.cn) |
| 英文摘要 | Aim: to study the role of dopamine (da) in rotenone-induced neurotoxicity in pc 12 cells. methods: cell viability was assessed by detecting the leakage of lactate dehydrogenase (ldh) into the medium. apoptosis rate was measured by flow cytometry. caspase-3-like activity was measured by fluorescence assay using the probe ac-devd-amc. the level of intracellular hydrogen peroxide and other peroxides in pc12 cells were quantified by loading cells with 2'-7'-dichlorodihydrofluorescein diacetate (dcfh-da) in fluorescence assay. lactic acid was measured spectrophotometrically. the da levels in pc12 cells were determined by hplc-ecd. results: a 48-h incubation of pc12 cells with rotenone caused an apoptotic cell death and elevated intracellular reactive oxygen species (ros) and lactic acid accumulation. intracellular da depletion with reserpine significantly attenuated rotenone-induced ros accumulation and apoptotic cell death. no change was found in rotenone-induced ros accumulation when cells were co-treated with deprenyl. brief treatment with reserpine at the end of rotenone treatment had no effect on rotenone-induced neurotoxicity. however, when cells were first incubated with deprenyl, a monoamine oxidase-b inhibitor for 30 min then co-incubated with rotenone plus deprenyl, a brief treatment with reserpine enhanced cell injury. conclusion: rotenone-induced apoptosis in pc12 cells was mediated by intracellular dopamine oxidation. |
| WOS关键词 | PARKINSONS-DISEASE ; ALPHA-SYNUCLEIN ; NITRIC-OXIDE ; HYDROGEN-PEROXIDE ; SUBSTANTIA-NIGRA ; NERVE-TERMINALS ; MITOCHONDRIA ; TOXICITY ; BRAIN ; NEUROTOXICITY |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000226426600003 |
| 出版者 | SHANGHAI INST MATERIA MEDICA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375236 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhu, XZ |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Mat Med, Dept Pharmacol, Shanghai 201203, Peoples R China 2.E China Normal Univ, Dept Environm Sci & Technol, Shanghai 200062, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, HQ,Zhu, XZ,Weng, EQ. Intracellular dopamine oxidation mediates rotenone-induced apoptosis in pc12 cells[J]. Acta pharmacologica sinica,2005,26(1):17-26. |
| APA | Liu, HQ,Zhu, XZ,&Weng, EQ.(2005).Intracellular dopamine oxidation mediates rotenone-induced apoptosis in pc12 cells.Acta pharmacologica sinica,26(1),17-26. |
| MLA | Liu, HQ,et al."Intracellular dopamine oxidation mediates rotenone-induced apoptosis in pc12 cells".Acta pharmacologica sinica 26.1(2005):17-26. |
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来源：武汉病毒研究所
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