Production of polyhedral inclusion bodies from helicoverpa armigera larvae infected with wild-type and recombinant hasnpv
文献类型：期刊论文
| 作者 | Sun, XL; Sun, XC; Bai, BK; Van der Werf, W; Vlak, JM; Hu, ZH |
| 刊名 | Biocontrol science and technology
 |
| 出版日期 | 2005-06-01 |
| 卷号 | 15期号:4页码:353-366 |
| 关键词 | Hasnpv Helicoverpa armigera Recombinants Pib production Larval instars |
| ISSN号 | 0958-3157 |
| DOI | 10.1080/09583150400016944 |
| 通讯作者 | Vlak, jm() |
| 英文摘要 | We report on the yield of polyhedral inclusion bodies ( pibs) from first to fifth instar helicoverpa armigera larvae inoculated with wild- type helicoverpa armigera nucleopolyhedrovirus ( hasnpv- wt) or with one of two hasnpv recombinants, one in which the ecdysteroid udp- glucosyltransferase ( egt) gene was deleted ( hasnpv- egtd) and a second in which the egt gene was replaced by a scorpion toxin ( aait) gene ( hasnpv- aait). a significant linear relationship between the logarithm of cadaver weight and the logarithm of the number of pibs per cadaver was observed for all three virus types. the increase of the number of pibs with larval weight was significantly greater for hasnpv- wt than for the recombinant viruses. for each of the three hasnpvs, pib yield per cadaver was significantly affected by larval instar at death and by time to death, with later instars and longer surviving larvae producing a greater number of pibs. as both recombinants caused host larvae to die at earlier instars than hasnpv- wt, their virus yields were significantly reduced. virus yield per larva, inoculated with hasnpv- aait in the first, second, third, fourth or fifth larval stage was 23, 32, 41, 44 and 47% of the yield of hasnpv- wt, respectively. for hasnpv- egtd, virus yield per larva inoculated in first through fifth instar, respectively, was 41, 55, 63, 54 and 82% of the yield of hasnpv- wt. these results provide a basis for optimizing the production regime of recombinant hasnpvs in larvae and for modeling the behavior of these viruses in agro- ecosystems. |
| WOS关键词 | SINGLE-NUCLEOCAPSID NUCLEOPOLYHEDROVIRUS ; TRICHOPLUSIA NI LARVAE ; PROCESS-BASED MODEL ; AUTOGRAPHA-CALIFORNICA ; BIOLOGICAL-CONTROL ; SPODOPTERA-EXIGUA ; BEET ARMYWORM ; BACULOVIRUSES ; HELIOTHIS ; GREENHOUSES |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Entomology |
| WOS类目 | Biotechnology & Applied Microbiology ; Entomology |
| 语种 | 英语 |
| WOS记录号 | WOS:000230082400003 |
| 出版者 | TAYLOR & FRANCIS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375238 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Vlak, JM |
| 作者单位 | 1.Univ Wageningen & Res Ctr, Virol Lab, NL-6709 PD Wageningen, Netherlands 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan, Hubei, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Mol Virol, Wuhan, Hubei, Peoples R China 4.Univ Wageningen & Res Ctr, Grp Crop & Weed Ecol, NL-6709 PD Wageningen, Netherlands |
| 推荐引用方式 GB/T 7714 | Sun, XL,Sun, XC,Bai, BK,et al. Production of polyhedral inclusion bodies from helicoverpa armigera larvae infected with wild-type and recombinant hasnpv[J]. Biocontrol science and technology,2005,15(4):353-366. |
| APA | Sun, XL,Sun, XC,Bai, BK,Van der Werf, W,Vlak, JM,&Hu, ZH.(2005).Production of polyhedral inclusion bodies from helicoverpa armigera larvae infected with wild-type and recombinant hasnpv.Biocontrol science and technology,15(4),353-366. |
| MLA | Sun, XL,et al."Production of polyhedral inclusion bodies from helicoverpa armigera larvae infected with wild-type and recombinant hasnpv".Biocontrol science and technology 15.4(2005):353-366. |
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来源：武汉病毒研究所
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