Cloning and sequence analysis of the phytoene synthase gene from a unicellular chlorophyte, dunaliella salina
文献类型：期刊论文
| 作者 | Yan, Y; Zhu, YH; Jiang, JG; Song, DL |
| 刊名 | Journal of agricultural and food chemistry
 |
| 出版日期 | 2005-03-09 |
| 卷号 | 53期号:5页码:1466-1469 |
| 关键词 | Dunaliella salina Phytoene synthase Sequence analysis Phylogenetic relationship |
| ISSN号 | 0021-8561 |
| DOI | 10.1021/jf048358s |
| 通讯作者 | Jiang, jg() |
| 英文摘要 | Phytoene synthase (psy) catalyzing the dimerization of two molecules of geranylgeranyl pyrophosphate (ggpp) to phytoene may be rate limiting for the synthesis of beta-carotene in dunaliella salina. to elucidate the carotenogenic pathway in d. salina, the complete psy gene was first isolated by genome walking technology and suppression pcr. subsequently, rt-pcr was performed to obtain the psy cdna of 1260 bp, which codes 420 amino acids. the psy gene of total length of 2982 bp was found to consist of five exons and four introns when compared with the cdna sequence. the d. salina psy amino acid has a 78-89% similarity with many other higher plants, but a phylogenic analysis shows a closer relationship between the microalgal and cyanobacterial psy. |
| WOS关键词 | CAROTENOID BIOSYNTHESIS ; ESCHERICHIA-COLI ; HAEMATOCOCCUS-PLUVIALIS ; FUNCTIONAL-ANALYSIS ; PLANTS ; ACCUMULATION ; EXPRESSION ; EVOLUTION ; BACTERIA ; PRODUCTS |
| WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
| WOS类目 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000227391300026 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375240 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Jiang, JG |
| 作者单位 | 1.S China Univ Technol, Coll Food & Biotechnol, Guangzhou 510640, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Y,Zhu, YH,Jiang, JG,et al. Cloning and sequence analysis of the phytoene synthase gene from a unicellular chlorophyte, dunaliella salina[J]. Journal of agricultural and food chemistry,2005,53(5):1466-1469. |
| APA | Yan, Y,Zhu, YH,Jiang, JG,&Song, DL.(2005).Cloning and sequence analysis of the phytoene synthase gene from a unicellular chlorophyte, dunaliella salina.Journal of agricultural and food chemistry,53(5),1466-1469. |
| MLA | Yan, Y,et al."Cloning and sequence analysis of the phytoene synthase gene from a unicellular chlorophyte, dunaliella salina".Journal of agricultural and food chemistry 53.5(2005):1466-1469. |
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来源：武汉病毒研究所
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