Transgenic paulownia expressing shiva-1 gene has increased resistance to paulownia witches' broom disease
文献类型：期刊论文
| 作者 | Du, T; Wang, Y; Hu, QX; Chen, J; Liu, S; Huang, WJ; Lin, ML |
| 刊名 | Journal of integrative plant biology
 |
| 出版日期 | 2005-12-01 |
| 卷号 | 47期号:12页码:1500-1506 |
| 关键词 | Agrobacterium tumefaciens Paulownia Paulownia witch's broom Phytoplasma Shiva-1 |
| ISSN号 | 1672-9072 |
| 通讯作者 | Lin, ml() |
| 英文摘要 | Stem segments from diseased paulownia tomentosa xp. fortunei and leaves from healthy control were transformed with the expression vector p438prsi via agrobacterium tumefaciens. the p43 8prsi vector contained shiva-1 gene, which encodes an antibacterial peptide under the control of a camv35s promoter. the regenerated plants from transformed explants were planted in a greenhouse and nursery. pcr and southern blotting analysis showed that the shiva-1 gene was successfully integrated into the paulownia genome. transcription of the integrated shiva-1 gene was confirmed by rt-pcr. bioassay in the green house and phytoplasma dna-dot blotting demonstrated that resistance to paulownia witch's broom disease (pwb) increased significantly in shiva-1 -transgenic paulownia. further investigations indicated that higher shiva-1 expression correlated with fewer phytoplasma and less symptoms in diseased transgenic paulownia. together, our findings strongly suggest that breeding shiva-1-paulownia is an effective strategy to control pwb disease. |
| WOS关键词 | ANTIBACTERIAL PEPTIDE ; PROMOTER ; PLANTS ; RICE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000233777200011 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375242 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Lin, ML |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, T,Wang, Y,Hu, QX,et al. Transgenic paulownia expressing shiva-1 gene has increased resistance to paulownia witches' broom disease[J]. Journal of integrative plant biology,2005,47(12):1500-1506. |
| APA | Du, T.,Wang, Y.,Hu, QX.,Chen, J.,Liu, S.,...&Lin, ML.(2005).Transgenic paulownia expressing shiva-1 gene has increased resistance to paulownia witches' broom disease.Journal of integrative plant biology,47(12),1500-1506. |
| MLA | Du, T,et al."Transgenic paulownia expressing shiva-1 gene has increased resistance to paulownia witches' broom disease".Journal of integrative plant biology 47.12(2005):1500-1506. |
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来源：武汉病毒研究所
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