Identification of functional domains required for hearnpv p10 filament formation
文献类型：期刊论文
| 作者 | Dong, CS; Li, D; Long, G; Deng, F; Wang, HL; Hu, ZH |
| 刊名 | Virology
 |
| 出版日期 | 2005-07-20 |
| 卷号 | 338期号:1页码:112-120 |
| 关键词 | Hearnpv P10 Green fluorescent protein Functional domain Filament structure |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2005.05.003 |
| 通讯作者 | Hu, zh() |
| 英文摘要 | Baculovirus encoded p10 form fibrillar structures in infected cells. we have tried to identify the functional domains for the p10 filament formation by green fluorescence protein (gfp) tag. the p10 gene of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (hearnpv) was the subject of these studies. different lengths of hearnpv p10 gene were constructed with gfp fused in frame to the c-terminus. the constructs were transfected into insect and mammalian cells and observed by confocal microscopy. the results indicated that the first n-terminal 66 amino acids, which form the complete coiled-coil domain, were necessary for the aggregation and formation of filament structures of hearnpv p10. the proline-rich region and the c-terminal positively charged amino acids were not necessary for the formation of fibrillar structure but had some impact on the shape of the fibrillar structures. no other baculoviral proteins were needed for the formation of p10 filament structures in transfected cells. (c) 2005 elsevier inc. all rights reserved. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; BACULOVIRUS P10 ; HELICOVERPA-ZEA ; PROTEIN ; GENOME ; NUCLEOPOLYHEDROVIRUS ; SEQUENCE ; CELLS ; GENE |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000230470400011 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375246 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Hu, ZH |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Key Lab Mol Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, CS,Li, D,Long, G,et al. Identification of functional domains required for hearnpv p10 filament formation[J]. Virology,2005,338(1):112-120. |
| APA | Dong, CS,Li, D,Long, G,Deng, F,Wang, HL,&Hu, ZH.(2005).Identification of functional domains required for hearnpv p10 filament formation.Virology,338(1),112-120. |
| MLA | Dong, CS,et al."Identification of functional domains required for hearnpv p10 filament formation".Virology 338.1(2005):112-120. |
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来源：武汉病毒研究所
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