Cgi-mir-92d indirectly regulates tnf expression by targeting cds region of lipopolysaccharide-induced tnf-alpha factor 3 (cglitaf3) in oyster crassostrea gigas
文献类型：期刊论文
| 作者 | Chen, Hao1,3; Jiang, Shuai1; Wang, Lin1,3; Wang, Lingling2; Wang, Hao1; Qiu, Limei1; Song, Linsheng2 |
| 刊名 | Fish & shellfish immunology
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 55页码:577-584 |
| ISSN号 | 1050-4648 |
| 关键词 | Oyster Immunomodulation Mir-17-92 family Tumor necrosis factor Lps-induced tnf-alpha factor Inflammation |
| DOI | 10.1016/j.fsi.2016.06.036 |
| 通讯作者 | Wang, lingling() ; Song, linsheng(lshsong@ms.qdio.ac.cn) |
| 英文摘要 | Tumor necrosis factor alpha (tnf-alpha) mediated inflammatory response plays indispensable roles in organisms defending against the invaded bacteria, during which micrornas have been found crucial by controlling multiple tnf-alpha-related genes. in the present study, cgi-mir-92d was annotated as a member of mir-17-92 family and could target the cds region of lipopolysaccharide (lps)-induced tnf-alpha factor (cglitaf3) in oyster crassostrea gigas. it was observed that cgi-mir-92d could be vigorously modulated by vibrio splendidus or lps stimulation while cglitaf3 altered oppositely. two putative binding sites of cgi-mir-92d were then found at cds region of cglitaf3. the interaction between cgi-mir-92d and cglitaf3 was subsequently verified both in vitro and in vivo. as a result, a significant decrease of cellular luminescence was observed in cglitaf3 luciferase reporter assay when cgi-mir-92d was overexpressed. the luminescent decrease was then recuperated when cgi-mir-92d inhibitor was co-transfected with mirna mimics. besides, cglitaf3 transcripts were significantly down-regulated when cgi-mir-92d was overexpressed in vivo during v. splendidus challenge. gain-of-function assay of cglitaf3 was then conducted in hek293t cells to verify its function. consequently, a significant increase of tnf-alpha was observed during the assay. at the meantime, cgtnf was also down-regulated in gain-of-function assay of cgi-mir-92 in vivo, which was a member of tnf superfamily in oysters which could be robustly induced after pathogen stimulation. together, these results verify the interaction between cglitaf3 and cgi-mir-92d, which might dedicate crucially in the repaid activation of cgtnf expression during inflammatory response of oysters. (c) 2016 elsevier ltd. all rights reserved. |
| WOS关键词 | NECROSIS-FACTOR-ALPHA ; PACIFIC OYSTER ; FACTOR LITAF ; MIR-17-SIMILAR-TO-92 FAMILY ; VIBRIO-SPLENDIDUS ; MICRORNA TARGETS ; GENE-EXPRESSION ; INNATE IMMUNITY ; CODING REGIONS ; IDENTIFICATION |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000381537000063 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375251 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Lingling; Song, Linsheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Hao,Jiang, Shuai,Wang, Lin,et al. Cgi-mir-92d indirectly regulates tnf expression by targeting cds region of lipopolysaccharide-induced tnf-alpha factor 3 (cglitaf3) in oyster crassostrea gigas[J]. Fish & shellfish immunology,2016,55:577-584. |
| APA | Chen, Hao.,Jiang, Shuai.,Wang, Lin.,Wang, Lingling.,Wang, Hao.,...&Song, Linsheng.(2016).Cgi-mir-92d indirectly regulates tnf expression by targeting cds region of lipopolysaccharide-induced tnf-alpha factor 3 (cglitaf3) in oyster crassostrea gigas.Fish & shellfish immunology,55,577-584. |
| MLA | Chen, Hao,et al."Cgi-mir-92d indirectly regulates tnf expression by targeting cds region of lipopolysaccharide-induced tnf-alpha factor 3 (cglitaf3) in oyster crassostrea gigas".Fish & shellfish immunology 55(2016):577-584. |
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来源：中国科学院大学
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