Transfer and expression of the mosquitocidal plasmid pbtoxis in bacillus cereus group strains
文献类型：期刊论文
| 作者 | Hu, XM; Hansen, BM; Yuan, ZM; Johansen, JE; Eilenberg, J; Hendriksen, NB; Smidt, L; Jensen, GB |
| 刊名 | Fems microbiology letters
 |
| 出版日期 | 2005-04-15 |
| 卷号 | 245期号:2页码:239-247 |
| 关键词 | Conjugation Pbtoxis Israelensis Lepidopteran Dipteran Mosquitocidal Transconjugant |
| ISSN号 | 0378-1097 |
| DOI | 10.1016/j.femsle.2005.03.012 |
| 通讯作者 | Yuan, zm() |
| 英文摘要 | The toxicity of bacillus thuringiensis subsp. israelensis to dipteran larvae (mosquitoes and black flies) depends on the presence of the pbtoxis plasmid. in this paper, two antibiotic resistance tagged pbtoxis were transferred by conjugation to other bacillus cereals group strains. among 15 potential recipients, only a lepidopteran active b. thuringiensis subspecies kurstaki and a b. cereus strain received the plasmid pbtoxis with a low transfer rate of about 10(-8) transconjugants/recipient. the resulting b. thuringiensis subspecies kurstaki transconjugant was active to both lepidopteran and dipteran targets and the b. cereals transconjugant was active against dipteran insects. phase contrast microscopy showed that the: b. cereus transconjugants could produce only round crystalline inclusion bodies while b. thuringiensis subspecies kurstaki transconjugant could produce both round and bipyramidal crystals during sporulation. sds-page revealed that all the major mosquitocidal proteins from pbtoxis could express in the two transconjugants, including cry4aa, cry4ba, cry10aa, cry11aa and cyt1aa. however, none of the experiment showed any indications of mobilising abilities of pbtoxis. the limited number of strains, which could receive and maintain pbtoxis using a conjugational helper plasmid, indicates a very narrow host range of the b. thuringiensis subsp. israelensis pbtoxis plasmid. (c) 2005 federation of european microbiological societies. published by elsevier b.v. all rights reserved. |
| WOS关键词 | THURINGIENSIS SUBSP ISRAELENSIS ; MEDIATED CONJUGATION SYSTEM ; TRANSMISSIBLE PLASMID ; B-THURINGIENSIS ; AGGREGATION ; GENES ; MOBILIZATION ; RESISTANCE ; SYNERGISM ; TOXICITY |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000228753100006 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375252 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yuan, ZM |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Natl Environm Res Inst, DK-4000 Roskilde, Denmark 3.Royal Vet & Agr Univ, DK-1871 Frederiksberg, Denmark 4.Natl Inst Occupat Hlth, DK-2100 Copenhagen, Denmark 5.Thomsen Biosci, DK-9400 Noerresundby, Denmark |
| 推荐引用方式 GB/T 7714 | Hu, XM,Hansen, BM,Yuan, ZM,et al. Transfer and expression of the mosquitocidal plasmid pbtoxis in bacillus cereus group strains[J]. Fems microbiology letters,2005,245(2):239-247. |
| APA | Hu, XM.,Hansen, BM.,Yuan, ZM.,Johansen, JE.,Eilenberg, J.,...&Jensen, GB.(2005).Transfer and expression of the mosquitocidal plasmid pbtoxis in bacillus cereus group strains.Fems microbiology letters,245(2),239-247. |
| MLA | Hu, XM,et al."Transfer and expression of the mosquitocidal plasmid pbtoxis in bacillus cereus group strains".Fems microbiology letters 245.2(2005):239-247. |
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来源：武汉病毒研究所
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