Observation of the interaction between muts and mutl mismatch repair proteins by fusion protein systems
文献类型：期刊论文
| 作者 | Bi, LJ; Zhang, XE; Zhou, YF; Zhang, ZP |
| 刊名 | Progress in biochemistry and biophysics
 |
| 出版日期 | 2005-12-01 |
| 卷号 | 32期号:12页码:1178-1184 |
| 关键词 | Fusion protein Gfp Strep tagii Muts Mutl Interaction |
| ISSN号 | 1000-3282 |
| 通讯作者 | Zhang, xe(zhangxe@mail.most.gov.cn) |
| 英文摘要 | Mutl and muts or their homologues are two crucial proteins of dna mismatch repair (nuvir) system. a new method was described for observation of the interaction between muts and mutl which is based on the fusion gene/fusion protein technique. three fusion proteins,. mutl-gfp fusion (trx-his(6)-gfp-(scr-gly)(6)-mutl), mutl-strep tag it fusion (trx-his(6)-(ser-gly)(6)-strep tagii-(ser-gly)(6)-mutl) and muts fusion (trx-his(6)-(ser-gly)(6)-muts), were constructed and expressed in e.coli ad494 (de3). interaction assay between muts and mutl was performed in a 96-well microtiter plate. muts fusion protein was immobilized on the wells and provided a surface for the interaction between muts and mutl. results showed that only after binding of muts to the mismatched dna, there was an interaction between muts and mutl. the binding events could be indicated by gfp signal or the signal generated from alkaline phosphatase and its substrate. in addition, the method based on fusion molecular system also serve as a model for studies on the interactions among other proteins or biomolecules. |
| WOS关键词 | ESCHERICHIA-COLI ; DNA MUTATIONS ; BINDING ; CONSTRUCTION ; CHIP |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000234090700011 |
| 出版者 | CHINESE ACAD SCIENCES, INST BIOPHYSICS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375256 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, XE |
| 作者单位 | 1.Chinese Acad Sci, Natl Lab Biomacromol, IBP WIV Joint Res Grp Analyt Biotechnol, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Natl Lab Virol, Wuhan Inst Virol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bi, LJ,Zhang, XE,Zhou, YF,et al. Observation of the interaction between muts and mutl mismatch repair proteins by fusion protein systems[J]. Progress in biochemistry and biophysics,2005,32(12):1178-1184. |
| APA | Bi, LJ,Zhang, XE,Zhou, YF,&Zhang, ZP.(2005).Observation of the interaction between muts and mutl mismatch repair proteins by fusion protein systems.Progress in biochemistry and biophysics,32(12),1178-1184. |
| MLA | Bi, LJ,et al."Observation of the interaction between muts and mutl mismatch repair proteins by fusion protein systems".Progress in biochemistry and biophysics 32.12(2005):1178-1184. |
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来源：武汉病毒研究所
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