Characterization of orf2 and its encoded protein of the helicoverpa armigera nucleopolyhedrovirus
文献类型：期刊论文
| 作者 | Nie, YC; Wang, Q; Liang, CY; Fang, MG; Yu, ZH; Chen, XW |
| 刊名 | Virus research
 |
| 出版日期 | 2006-03-01 |
| 卷号 | 116期号:1-2页码:129-135 |
| 关键词 | Hasnpv P78/83 Ha2 Wasp Baculovirus Structural protein |
| ISSN号 | 0168-1702 |
| DOI | 10.1016/j.virusres.2005.09.007 |
| 通讯作者 | Chen, xw() |
| 英文摘要 | The open reading frame 2 (ha2) of the helicoverpa armigera single nucleocaspid nucleopolyhedrovirus (hasnpv), a conserved gene in 111ost baculoviruses from lepidopteran insects such as p78/83 of the autographa californica mnpv, was characterized. it is 1242 bp long and potentially encodes a 45.9 kda. ha2 is conserved among baculoviruses from lepicdopteran insects. ha2 transcripts were detected front 16 to 96 11 post infection (hpi) of hzam1 cells. rabbit polyclonal antiserum against a gst-ha2 fusion protein reacted with three protein of 50, 46 and 35 kda at 24-72 hpi of hzam1 cells. anti opmnpv orf2 (homologue of ha2) antibody reacted only with the 46 and 35 kda proteins in hasnpv-infected cells. these results demonstrate that ha2 is modified at the mrna or protein levels. western blot analysis showed that only the 50kda product of ha2 is a structural component of proteins of both the budded virus (bv) and occlusion-derived virus (odv) phenotypes. ha2-egfp fusion protein showed that ha2 is localized primarily in the nucleus of hzam1 infected cells. the ha2 was found to co-localize with actin by labelling of actin with rhordamine-phalloidin. in summary, the data indicated that ha2 is a structural protein and interacts with host cell actin. (c) 2005 elsevier b.v. all rights reserved. |
| WOS关键词 | ACTIN-BASED MOTILITY ; VACCINIA VIRUS ; NEW-GENERATION ; BACULOVIRUS ; CORTACTIN ; CYTOSKELETON ; NUCLEATION ; PHYLOGENY ; PATHOGENS ; SEQUENCES |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000235864400016 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375271 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, XW |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Cent China Normal Univ, Inst Entomol, Wuhan 430070, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Nie, YC,Wang, Q,Liang, CY,et al. Characterization of orf2 and its encoded protein of the helicoverpa armigera nucleopolyhedrovirus[J]. Virus research,2006,116(1-2):129-135. |
| APA | Nie, YC,Wang, Q,Liang, CY,Fang, MG,Yu, ZH,&Chen, XW.(2006).Characterization of orf2 and its encoded protein of the helicoverpa armigera nucleopolyhedrovirus.Virus research,116(1-2),129-135. |
| MLA | Nie, YC,et al."Characterization of orf2 and its encoded protein of the helicoverpa armigera nucleopolyhedrovirus".Virus research 116.1-2(2006):129-135. |
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来源：武汉病毒研究所
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