Rapid fluorometric screening of antibiotics in seafood
文献类型：期刊论文
| 作者 | Liu, Y; Danielsson, B |
| 刊名 | Microchimica acta
 |
| 出版日期 | 2006-02-01 |
| 卷号 | 153期号:3-4页码:133-137 |
| 关键词 | Chloramphenicol Seafood contamination Sulfathiazole Dna-based drug screening Fluorometric screening |
| ISSN号 | 0026-3672 |
| DOI | 10.1007/s00604-005-0421-9 |
| 通讯作者 | Danielsson, b() |
| 英文摘要 | Simple and rapid fluorometric screening methods have been developed based on the competitive binding between the target and an intercalating fluorophore dye to double-stranded-dna (dsdna). in this study, the long-wavelength fluorescente dye toto-3 was employed as the indicator. compounds that interact with dsdna will affect the binding of toto-3 to the nucleic acid thereby changing the fluorescence intensity. the analyte concentration is indirectly determined by the decrease in fluorescence intensity. a fiber optic fluorescence screening system was developed for rapid and convenient sample processing. lambda dna (48.5kb) was chosen as a suitable sensing nucleic acid material. detection of sulfathiazole and chloramphenicol in shrimps using this method was studied in the range of 0.5-25ng ml(-1) of sulfathiazole and of 1-50ng ml(-1) of chloramphenicol. detection limits of 0.5ng ml(-1) of sulfathiazole and 1ng ml(-1) of chloramphenicol were achieved. this approach is useful as a routine test in the monitoring of antibiotics in the environment or aquaculture products. the easy operation and the rapid and sensitive detection make this a potential high-throughput screening method. |
| WOS关键词 | DNA ELECTROCHEMICAL BIOSENSORS ; POLYMERASE-CHAIN-REACTION ; AFFINITY ; IMMOBILIZATION |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000235202500003 |
| 出版者 | SPRINGER WIEN |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375303 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Danielsson, B |
| 作者单位 | 1.Lund Univ, SE-22100 Lund, Sweden 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Y,Danielsson, B. Rapid fluorometric screening of antibiotics in seafood[J]. Microchimica acta,2006,153(3-4):133-137. |
| APA | Liu, Y,&Danielsson, B.(2006).Rapid fluorometric screening of antibiotics in seafood.Microchimica acta,153(3-4),133-137. |
| MLA | Liu, Y,et al."Rapid fluorometric screening of antibiotics in seafood".Microchimica acta 153.3-4(2006):133-137. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。