Construction of three single-chain antibody fragment variable fusion structures for sensitive detection of nucleocapsid protein of hantaan virus
文献类型：期刊论文
| 作者 | Chen, Jia; Xie, Wei-Hong; Zhang, Zhi-Ping; Zhang, Xian-En; Li, Wei; Wang, Shi-Hua; Zhou, Ya-Feng; Liang, Mi-Fang; Wang, Ke-Min; Yang, Zhan-Qiu |
| 刊名 | Analytical letters
 |
| 出版日期 | 2006 |
| 卷号 | 39期号:10页码:2153-2167 |
| 关键词 | Hantaan virus Single-chain antibody fragment variable (scfv) Fusion protein Alkaline phosphatase Fluorescence dye Nanoparticles |
| ISSN号 | 0003-2719 |
| DOI | 10.1080/0032710600748905 |
| 通讯作者 | Zhang, xian-en(zhangxe@mail.most.gov.cn) |
| 英文摘要 | Three single-chain fragment variable (scfv) fusion structures were constructed for use in rapid and sensitive detection of nucleocapsid protein (np) of hantaan virus. the detection of nps on glass chips was signalized by enzyme labeling or fluorescence dye cy3, or cy5 cluster nanoparticles. the sensitivity of the methods with different signal systems was evaluated and compared. the detection limits of scfv-alkaline phosphatase fusion, fluorescence labeling (scfv-cy3), and nanoparticles labeling (scfv-sbp-streptavidin-nanoparticle) were 0.1 mu g/ml, 1 ng/ml, and 0.1 ng/ml np, respectively, which were all lower than that in a conventional enzyme-linked immunosorbent assay (elisa) (1 mu g/ml). twenty hantaan virus isolates were detected using the proposed methods. |
| WOS关键词 | DOPED SILICA NANOPARTICLES ; LINKED-IMMUNOSORBENT-ASSAY ; ESCHERICHIA-COLI ; NEPHROPATHIA-EPIDEMICA ; HANTAVIRUS INFECTIONS ; HEMORRHAGIC-FEVER ; RENAL SYNDROME ; DNA MUTATIONS ; RT-PCR ; RECOMBINANT |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000239384600002 |
| 出版者 | TAYLOR & FRANCIS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375304 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Xian-En |
| 作者单位 | 1.Chinese Acad Sci, Joint Res Grp Analyt Pathogen Microbiol, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Inst Biophys, State Key Lab Macromol, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, Beijing, Peoples R China 5.Hunan Univ, State Key Lab Chem & Biol Sensing & Chemometr, Changsha, Peoples R China 6.Wuhan Univ, Inst Virol, Coll Med, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Jia,Xie, Wei-Hong,Zhang, Zhi-Ping,et al. Construction of three single-chain antibody fragment variable fusion structures for sensitive detection of nucleocapsid protein of hantaan virus[J]. Analytical letters,2006,39(10):2153-2167. |
| APA | Chen, Jia.,Xie, Wei-Hong.,Zhang, Zhi-Ping.,Zhang, Xian-En.,Li, Wei.,...&Yang, Zhan-Qiu.(2006).Construction of three single-chain antibody fragment variable fusion structures for sensitive detection of nucleocapsid protein of hantaan virus.Analytical letters,39(10),2153-2167. |
| MLA | Chen, Jia,et al."Construction of three single-chain antibody fragment variable fusion structures for sensitive detection of nucleocapsid protein of hantaan virus".Analytical letters 39.10(2006):2153-2167. |
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来源：武汉病毒研究所
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