Preliminary characterization of a thermostable dna polymerase i from a mesophilic bacillus sphaericus strain c3-41
文献类型：期刊论文
| 作者 | Han Bei; Liu Haizhou; Hu Xiaomin; Yuan Zhiming |
| 刊名 | Archives of microbiology
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 186期号:3页码:203-209 |
| 关键词 | Thermostable dna polymerase i Bacillus sphaericus Mesophile Mosquitocidal activity |
| ISSN号 | 0302-8933 |
| DOI | 10.1007/s00203-006-0135-3 |
| 通讯作者 | Yuan zhiming(yzm@pentium.whiov.ac.cn) |
| 英文摘要 | A thermostable dna polymerase i from a mesophilic bacillus sphaericus strain c3-41 was characterized in this study. the poli was cloned, sequenced and over-expressed in escherichia coli. the expressed 110 kda fusion protein of poli was stable at 70 degrees c for 1 h. compared with dna polymerase i of e. coli (takara), the relative polymerase activity of this poli was 3.33 +/- 0.1 rfu mu l(-1) at 37 degrees c using fluorescent quantitative analysis. it showed higher polymerase activity than e. coli poli at higher temperature, with a relative activity of 3.75 +/- 0.1 rfu mu l(-1) at 70 degrees c. the poli sequence analysis and the protein structure prediction indicated that this protein had a high similarly to other poli from thermophilic micro-organisms. this information is of importance for future study for evolution of the house-keeping gene poli in entomopathogenic bacterium b. sphaericus. |
| WOS关键词 | REVERSE-TRANSCRIPTASE ; THERMUS-AQUATICUS ; PROTEINS ; IDENTIFICATION ; EXPRESSION ; TOXIN |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000239956300005 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375310 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yuan Zhiming |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han Bei,Liu Haizhou,Hu Xiaomin,et al. Preliminary characterization of a thermostable dna polymerase i from a mesophilic bacillus sphaericus strain c3-41[J]. Archives of microbiology,2006,186(3):203-209. |
| APA | Han Bei,Liu Haizhou,Hu Xiaomin,&Yuan Zhiming.(2006).Preliminary characterization of a thermostable dna polymerase i from a mesophilic bacillus sphaericus strain c3-41.Archives of microbiology,186(3),203-209. |
| MLA | Han Bei,et al."Preliminary characterization of a thermostable dna polymerase i from a mesophilic bacillus sphaericus strain c3-41".Archives of microbiology 186.3(2006):203-209. |
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来源：武汉病毒研究所
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