Molecular cloning and heterologous expression of an alkaline xylanase from bacillus pumilus hbp8 in pichia pastoris
文献类型：期刊论文
| 作者 | Zhang, Gui-Min; Hu, Yong; Zhuang, Yong-Hong; Ma, Li-Xin; Zhang, Xian-En |
| 刊名 | Biocatalysis and biotransformation
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 24期号:5页码:371-379 |
| 关键词 | Bacillus pumilus hbp8 Cloning Deglycosylation Heterologous expression Pichia pastoris Xylanase |
| ISSN号 | 1024-2422 |
| DOI | 10.1020/10242420600768771 |
| 通讯作者 | Ma, li-xin(malixing@hubu.edu.cn) |
| 英文摘要 | The xynhb gene, encoding alkaline xylanase was cloned from bacillus pumilus by a shot-gun method. the gene was cloned into vector phbm905a, and expressed in pichia pastoris gs115. xylanase-secreting transformants were selected on plates containing rbb-xylan. enzymatic activity in the culture supernatants was up to 644 u ml(-1) and the optimal secretion time was 4 days at 25 degrees c. sds-page showed two bands, of 32.2 kda and 29.6 kda, both larger than the predicted mass of 22.4 kda based on its amino acid sequence. zymogram analysis demonstrated that the enzyme in both bands could hydrolyze xylan. deglycosylation by endoglycosidase h revealed that both were derived from the same protein but contain different extents of glycosylation (30 and 25%). the optimal ph and temperature of the enzyme was ph6-9 and 50 degrees c, respectively. |
| WOS关键词 | SACCHAROMYCES-CEREVISIAE ; GENE ; XYNA ; GLYCOSYLATION ; SECRETION ; SEQUENCE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000242538000005 |
| 出版者 | TAYLOR & FRANCIS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375312 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Ma, Li-Xin |
| 作者单位 | 1.Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China 2.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Gui-Min,Hu, Yong,Zhuang, Yong-Hong,et al. Molecular cloning and heterologous expression of an alkaline xylanase from bacillus pumilus hbp8 in pichia pastoris[J]. Biocatalysis and biotransformation,2006,24(5):371-379. |
| APA | Zhang, Gui-Min,Hu, Yong,Zhuang, Yong-Hong,Ma, Li-Xin,&Zhang, Xian-En.(2006).Molecular cloning and heterologous expression of an alkaline xylanase from bacillus pumilus hbp8 in pichia pastoris.Biocatalysis and biotransformation,24(5),371-379. |
| MLA | Zhang, Gui-Min,et al."Molecular cloning and heterologous expression of an alkaline xylanase from bacillus pumilus hbp8 in pichia pastoris".Biocatalysis and biotransformation 24.5(2006):371-379. |
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来源：武汉病毒研究所
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