Site-directed mutagenesis of gentisate 1,2-dioxygenases from klebsiella pneumoniae m5a1 and ralstonia sp strain u2
文献类型：期刊论文
| 作者 | Luo, S; Liu, DQ; Liu, H; Zhou, NY |
| 刊名 | Microbiological research
 |
| 出版日期 | 2006 |
| 卷号 | 161期号:2页码:138-144 |
| 关键词 | Gentisate 1,2-dixoygenase Klebsiella pneumoniae m5a1 Ralstonia sp strain u2 Site-directed mutagenesis |
| ISSN号 | 0944-5013 |
| DOI | 10.1016/j.micres.2005.07.004 |
| 通讯作者 | Zhou, ny() |
| 英文摘要 | Gentisate 1,2-dioxygenase (gdo, ec 1.13.11.4) is the first enzyme in gentisate pathway that catalyses the ring fission of gentisate to form maleylpyruvate. phytogenetic tree of amino acid sequences from 11 gdos demonstrates that the gdos from different genus share identities between 12.1% and 64.8%. according to the alignment result, four highly conserved histidine residues in gdo from klebsiella pneumoniae m5a1 and ralstonia sp, strain u2 were chosen to be substituted with aspartate residues. enzyme analysis indicated that substitution of any of these four histidine residues had resulted in the complete loss of its catalytic activity. (c) 2005 elsevier gmbh. all rights reserved. |
| WOS关键词 | CLEAVING CATECHOLIC DIOXYGENASE ; CRYSTAL-STRUCTURE ; PSEUDOMONAS ; PURIFICATION ; PROTEINS ; 2,3-DIOXYGENASE ; IDENTIFICATION ; COORDINATION ; SUPERFAMILY ; EVOLUTION |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000235609300007 |
| 出版者 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375314 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhou, NY |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, S,Liu, DQ,Liu, H,et al. Site-directed mutagenesis of gentisate 1,2-dioxygenases from klebsiella pneumoniae m5a1 and ralstonia sp strain u2[J]. Microbiological research,2006,161(2):138-144. |
| APA | Luo, S,Liu, DQ,Liu, H,&Zhou, NY.(2006).Site-directed mutagenesis of gentisate 1,2-dioxygenases from klebsiella pneumoniae m5a1 and ralstonia sp strain u2.Microbiological research,161(2),138-144. |
| MLA | Luo, S,et al."Site-directed mutagenesis of gentisate 1,2-dioxygenases from klebsiella pneumoniae m5a1 and ralstonia sp strain u2".Microbiological research 161.2(2006):138-144. |
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来源：武汉病毒研究所
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