Potential role for p53 in the permissive life cycle of human cytomegalovirus
文献类型：期刊论文
| 作者 | Casavant, N. C.; Luo, A. H.; Rosenke, K.; Winegardner, T.; Zurawska, A.; Fortunato, E. A. |
| 刊名 | Journal of virology
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 80期号:17页码:8390-8401 |
| ISSN号 | 0022-538X |
| DOI | 10.1128/jvi.00505-06 |
| 通讯作者 | Fortunato, e. a.(lfort@uidaho.edu) |
| 英文摘要 | Infection of primary fibroblasts with human cytomegalovirus (hcmv) causes a rapid stabilization of the cellular protein p53. p53 is a major effector of the cellular damage response, and activation of this transcription factor can lead either to cell cycle arrest or to apoptosis. viruses employ many tactics to avoid p53-mediated effects. one method hcmv uses to counteract p53 is sequestration into its viral replication centers. in order to determine whether or not hcmv benefits from this sequestration, we infected a p53(-/-) fibroblast line. we find that although these cells are permissive for viral infection, several parameters are substantially altered compared to wild-type (wt) fibroblasts. p53(-/-) cells show delayed and decreased accumulation of infectious viral particles compared to control fibroblasts, with the largest difference of 100-fold at 72 h post infection (p.i.) and peak titers decreased by approximately 10- to 20-fold at 144 h p.i. viral dna accumulation is also delayed and somewhat decreased in p53(-/-) cells; however, on average, levels of dna are not more than fivefold lower than wt at any time p.i. and thus cannot account entirely for the observed differences in titers. in addition, there are delays in the expression of several key viral proteins, including the early replication protein ul44 and some of the late structural proteins, pp28 (ul99) and mcp (ul86). ul44 localization also indicates delayed formation and maturation of the replication centers throughout the course of infection. localization of the major tegument protein pp65 (ul83) is also altered in these p53(-/-) cells. partial reconstitution of the p53(-/-) cells with a wt copy of p53 returns all parameters toward wt, while reconstitution with mutant p53 does not. taken together, our data suggest that wt p53 enhances the ability of hcmv to replicate and produce high concentrations of infectious virions in permissive cells. |
| WOS关键词 | WILD-TYPE P53 ; HUMAN-PAPILLOMAVIRUS TYPE-16 ; IMMEDIATE-EARLY PROTEINS ; VIRAL REPLICATION ; DNA-BINDING ; TRANSCRIPTIONAL REPRESSION ; COMPLEX-FORMATION ; GENE-EXPRESSION ; IE86 PROTEIN ; S-PHASE |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000239934500010 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375315 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Fortunato, E. A. |
| 作者单位 | 1.Univ Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USA 2.Univ Idaho, Ctr Reprod Biol, Moscow, ID 83844 USA 3.Acad Sinica, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Casavant, N. C.,Luo, A. H.,Rosenke, K.,et al. Potential role for p53 in the permissive life cycle of human cytomegalovirus[J]. Journal of virology,2006,80(17):8390-8401. |
| APA | Casavant, N. C.,Luo, A. H.,Rosenke, K.,Winegardner, T.,Zurawska, A.,&Fortunato, E. A..(2006).Potential role for p53 in the permissive life cycle of human cytomegalovirus.Journal of virology,80(17),8390-8401. |
| MLA | Casavant, N. C.,et al."Potential role for p53 in the permissive life cycle of human cytomegalovirus".Journal of virology 80.17(2006):8390-8401. |
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来源：武汉病毒研究所
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