Protection against avian influenza h9n2 virus challenge by immunization with hemagglutinin- or neuraminidase-expressing dna in balb/c mice
文献类型：期刊论文
| 作者 | Qiu, MZ; Fang, F; Chen, Y; Wang, HL; Chen, QJ; Chang, HY; Wang, FY; Wang, HZ; Zhang, R; Chen, Z |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2006-05-19 |
| 卷号 | 343期号:4页码:1124-1131 |
| ISSN号 | 0006-291X |
| 关键词 | Avian influenza Hemagglutinin Neuraminidase Dna vaccine |
| DOI | 10.1016/j.bbrc.2006.03.088 |
| 通讯作者 | Chen, z() |
| 英文摘要 | Avian influenza viruses of h9n2 subtype are widely spread in avian species. the viruses have recently been transmitted to mammalian species, including humans, accelerating the efforts to devise protective strategies against them. in this study, an avian influenza h9n2 virus strain (a/chicken/jiangsu/7/2002), isolated in jiangsu province, china, was used to infect balb/c mice for adaptation. after five lung-to-lung passages, the virus was stably proliferated in a large quantity in the murine lung and caused the deaths of mice. in addition, we explored the protection induced by h9n2 virus hemagglutinin (ha)- and neuraminidase (na)-expressing dnas in balb/c mice. female balb/c mice aged 6-8 weeks were immunized once or twice at a 3-week interval with ha-dna and na-dna by electroporation, respectively, each at a dose of 3, 10 or 30 mu g. the mice were challenged with a lethal dose (40x ld50) of influenza h9n2 virus four weeks after immunization once or one week after immunization twice. the protections of dna vaccines were evaluated by the serum antibody titers, residual lung virus titers, and survival rates of the mice. the result showed that immunization once with not less than 10 mu g or twice with 3 mu g ha-dna or na-dna provided effective protection against homologous avian influenza h9n2 virus. (c) 2006 elsevier inc. all rights reserved. |
| WOS关键词 | A VIRUS ; LETHAL INFLUENZA ; CROSS-PROTECTION ; SOUTHERN CHINA ; H5N1 VIRUSES ; HONG-KONG ; INFECTION ; VACCINE ; ELECTROPORATION ; IMMUNITY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| WOS记录号 | WOS:000237269000020 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375321 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Z |
| 作者单位 | 1.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 3.Shanghai Inst Biol Prod, Shanghai 200052, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, MZ,Fang, F,Chen, Y,et al. Protection against avian influenza h9n2 virus challenge by immunization with hemagglutinin- or neuraminidase-expressing dna in balb/c mice[J]. Biochemical and biophysical research communications,2006,343(4):1124-1131. |
| APA | Qiu, MZ.,Fang, F.,Chen, Y.,Wang, HL.,Chen, QJ.,...&Chen, Z.(2006).Protection against avian influenza h9n2 virus challenge by immunization with hemagglutinin- or neuraminidase-expressing dna in balb/c mice.Biochemical and biophysical research communications,343(4),1124-1131. |
| MLA | Qiu, MZ,et al."Protection against avian influenza h9n2 virus challenge by immunization with hemagglutinin- or neuraminidase-expressing dna in balb/c mice".Biochemical and biophysical research communications 343.4(2006):1124-1131. |
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来源：武汉病毒研究所
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