Expression of a ribosome-inactivating protein gene in bitter melon is induced by sphaerotheca fuliginea and abiotic stimuli
文献类型：期刊论文
| 作者 | Xu, Jun; Wang, Hualin; Fan, Jianming |
| 刊名 | Biotechnology letters
 |
| 出版日期 | 2007-10-01 |
| 卷号 | 29期号:10页码:1605-1610 |
| 关键词 | Bitter melon Fungal infection Momordica charantia Ribosome-inactivating protein Sphaerotheca |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-007-9433-3 |
| 通讯作者 | Fan, jianming(5746067@sohu.com) |
| 英文摘要 | The gene encoding a single-chain, ribosome-inactivating protein (scrip) was cloned from bitter melon (momordica charantia l.) leaves infected with the fungus, sphaerotheca fuliginea, by rt-pcr. the orf was 861 bp. the ribosome-inactivating protein was expressed in e. coli and, when purified, it inhibited the growth of the sphaerotheca fuliginea in vitro. northern blot analysis revealed that rip transcripts rapidly accumulated in leaves 1-day post inoculation with sphaerotheca fuliginea and reached a peak at 3 d. the expression pattern of rip induced by methyl jasmonate and salicylic acid were different from that of pathogen-induced expression. mechanical wounding, silver nitrate and osmotic stress stimulated only a slight accumulation of rip transcripts. abscisic acid also induced transcription of rips. the signal compounds, ethylene and okadaic acid, induced a moderate accumulation of rip transcripts. |
| WOS关键词 | DISEASE RESISTANCE ; ABSCISIC-ACID ; PLANTS ; JASMONATE ; INFECTION ; INDUCTION ; STRESS |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000248940500024 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375339 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Fan, Jianming |
| 作者单位 | 1.Zhengzhou Univ, Coll Publ Hlth, Toxicol Lab, Zhengzhou 450052, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Jun,Wang, Hualin,Fan, Jianming. Expression of a ribosome-inactivating protein gene in bitter melon is induced by sphaerotheca fuliginea and abiotic stimuli[J]. Biotechnology letters,2007,29(10):1605-1610. |
| APA | Xu, Jun,Wang, Hualin,&Fan, Jianming.(2007).Expression of a ribosome-inactivating protein gene in bitter melon is induced by sphaerotheca fuliginea and abiotic stimuli.Biotechnology letters,29(10),1605-1610. |
| MLA | Xu, Jun,et al."Expression of a ribosome-inactivating protein gene in bitter melon is induced by sphaerotheca fuliginea and abiotic stimuli".Biotechnology letters 29.10(2007):1605-1610. |
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来源：武汉病毒研究所
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