Immunogenicity comparison between codon optimized hiv-1crf bc_07 gp140 and gp145 vaccines
文献类型：期刊论文
| 作者 | Liu, Lianxing; Wan, Yanmin; Xu, Jianqing; Huang, Xianggang; Wu, Lan; Liu, Yong; Shao, Yiming |
| 刊名 | Aids research and human retroviruses
 |
| 出版日期 | 2007-11-01 |
| 卷号 | 23期号:11页码:1396-1404 |
| ISSN号 | 0889-2229 |
| DOI | 10.1089/aid.2007.0131 |
| 通讯作者 | Xu, jianqing(jianqingxu@chinaaids.cn) |
| 英文摘要 | To develop an effective vaccine against the most prevalent hiv strain "b '/c recombinant" in china, we compared the immunogenicity of b '/c-derived gp140 and gp145. the codon optimized gp140 and gp145 env gene derived from cn54, an ancestor-like b '/c recombinant strain, were synthesized and cloned into a plasmid as dna vaccines, designated as pdrvisv140 and pdrvisv145, respectively. balb/c mice were inoculated three times at week 0, 2, and 4 and sacrificed at week 7. both t cell immunity and humoral immunity were determined. the mock vector pdrvisv1.0 carrying no hiv immunogen was included as control. our data showed that b '/c recombinant-derived gp145 mounted stronger t cell and broader linear antibody but less binding antibody immune responses than gp140 did. though both gp145 and gp140 raised neutralization antibodies against laboratory-adapted strain sf33, both failed to neutralize b ' or b '/c clade primary strains. overall, this is the first time the immunogenicity of b '/c recombinant-derived gp140 and gp145 was examined and compared; our data prefer b '/c-derived gp145 to gp140 as an hiv vaccine immunogen. the failure to induce neutralization antibodies against primary isolates indicates that it is insufficient to enhance the immunogenicity of conserved epitopes by simply employing gp145 or gp140; strategies to enhance antibody responses against conserved epitopes should be explored further. |
| WOS关键词 | HUMAN-IMMUNODEFICIENCY-VIRUS ; TYPE-1 ENVELOPE GLYCOPROTEINS ; CANDIDATE AIDS VACCINES ; NEUTRALIZING ANTIBODIES ; MONOCLONAL-ANTIBODIES ; SURFACE GLYCOPROTEIN ; RECOMBINANT GP120 ; IMMUNE-RESPONSES ; TRIMERIC FORM ; V3 LOOP |
| WOS研究方向 | Immunology ; Infectious Diseases ; Virology |
| WOS类目 | Immunology ; Infectious Diseases ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000251538800013 |
| 出版者 | MARY ANN LIEBERT INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375342 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Xu, Jianqing |
| 作者单位 | 1.Chinese Ctr Dis Control & Prevent, Natl Ctr AIDS STD Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 100050, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Lianxing,Wan, Yanmin,Xu, Jianqing,et al. Immunogenicity comparison between codon optimized hiv-1crf bc_07 gp140 and gp145 vaccines[J]. Aids research and human retroviruses,2007,23(11):1396-1404. |
| APA | Liu, Lianxing.,Wan, Yanmin.,Xu, Jianqing.,Huang, Xianggang.,Wu, Lan.,...&Shao, Yiming.(2007).Immunogenicity comparison between codon optimized hiv-1crf bc_07 gp140 and gp145 vaccines.Aids research and human retroviruses,23(11),1396-1404. |
| MLA | Liu, Lianxing,et al."Immunogenicity comparison between codon optimized hiv-1crf bc_07 gp140 and gp145 vaccines".Aids research and human retroviruses 23.11(2007):1396-1404. |
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来源：武汉病毒研究所
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