The flhdc gene affects motility and biofilm formation in yersinia pseudotuberculosis (retracted article. see vol. 52, pg. 796, 2009)
文献类型：期刊论文
| 作者 | Yao, Wang; Lisha, Ding; YangBo, Hu; Yong, Zhang; BaoYu, Yang; ShiYun, Chen |
| 刊名 | Science in china series c-life sciences
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 50期号:6页码:814-821 |
| 关键词 | Yersinia pseudotuberculosis Flhdc Motility Biofilm |
| ISSN号 | 1006-9305 |
| DOI | 10.1007/s11427-007-0101-6 |
| 通讯作者 | Shiyun, chen(ychen@wh.iov.cn) |
| 英文摘要 | The flagella master regulatory gene flhdc of yersinia pseudotuberculosis serotype iii (ypiii) was mutated by deleting the middle region and replaced by a tetracycline resistant gene, and the subsequent mutant strain named ypiii. flhdc was obtained. swimming assay showed that the swimming motility of the mutant strain was completely abolished. the promoter region of the flagella second-class regulatory gene flia was fused with the lux box, and was conjugated with the mutant and the parent strains respectively for the first cross. lucy assay result demonstrated that flhdc regulated the expression of flia in ypiii as reported in e. coli. biofilm formation of the mutant strain on abiotic and biotic surfaces was observed and quantified. the results showed that mutation of flhdc decreased biofilm formation on both abiotic and biotic surfaces, and abated the infection on caenorhabdtis elegans. our results suggest that mutation of the flagella master regulatory gene flhdc not only abolished the swimming motility, but also affected biofilm formation of ypiii on different surfaces. the new function of flhdc identified in this study provides a novel viewpoint for the control of bacterial biofilm formation. |
| WOS关键词 | FLAGELLAR MASTER OPERON ; QUORUM-SENSING SYSTEM ; PSEUDOMONAS-AERUGINOSA ; ESCHERICHIA-COLI ; CAENORHABDITIS-ELEGANS ; VIRULENCE ; EXPRESSION ; PHOSPHOLIPASE ; REGULATOR ; COMPLEX |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| WOS类目 | Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000251020100014 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375344 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | ShiYun, Chen |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Wang,Lisha, Ding,YangBo, Hu,et al. The flhdc gene affects motility and biofilm formation in yersinia pseudotuberculosis (retracted article. see vol. 52, pg. 796, 2009)[J]. Science in china series c-life sciences,2007,50(6):814-821. |
| APA | Yao, Wang,Lisha, Ding,YangBo, Hu,Yong, Zhang,BaoYu, Yang,&ShiYun, Chen.(2007).The flhdc gene affects motility and biofilm formation in yersinia pseudotuberculosis (retracted article. see vol. 52, pg. 796, 2009).Science in china series c-life sciences,50(6),814-821. |
| MLA | Yao, Wang,et al."The flhdc gene affects motility and biofilm formation in yersinia pseudotuberculosis (retracted article. see vol. 52, pg. 796, 2009)".Science in china series c-life sciences 50.6(2007):814-821. |
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来源：武汉病毒研究所
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