High-mannose-specific deglycosylation of hiv-1 gp120 induced by resistance to cyanovirin-n and the impact on antibody neutralization
文献类型：期刊论文
| 作者 | Hu, Qinxue; Mahmood, Naheed; Shattock, Robin J. |
| 刊名 | Virology
 |
| 出版日期 | 2007-11-10 |
| 卷号 | 368期号:1页码:145-154 |
| 关键词 | Hiv-1 Envelope glycoprotein Carbohydrate-binding agent Cyanovirin-n Resistance Antibody Neutralization V3 loop |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2007.06.029 |
| 通讯作者 | Shattock, robin j.(shattock@sgul.ac.uk) |
| 英文摘要 | Hiv-1 uses glycans on gp 120 to occlude its highly immunogenic epitopes. to better elucidate escape mechanisms of hiv-1 from carbohydrate-binding agents (cba) and to understand the impact of cba-escape on viral immune evasion, we generated and examined the biological properties of hiv-1 resistant to cyanovirin-n (cv-n) or cross-resistant to additional cbas. genotypic and phenotypic characterization of resistant env clones indicated that 3-5 high-mannose residues from 289 to 448 in the c2-c4 region of gp 120 were mutated and correlated with the resistance levels. the specificity and minimal requirements of deglycosylation for cv-n resistance were further assessed by mutagenesis study. the sensitivity of resistant variants to a range of cbas, immunoglobulins, sera and monoclonal antibodies (mab) were investigated. for the first time, our data have collectively defined the high-mannose residues on gp120 affecting cv-n activity, and demonstrated that cba-escape hiv-1 has increased sensitivity to immunoglobulins and sera from hiv patients, and particularly to v3 loop-directed mabs. our study provides a proof-of-concept that targeting hiv-1 glycan shields may represent a novel antiviral strategy. (c) 2007 elsevier inc. all rights reserved. |
| WOS关键词 | IMMUNODEFICIENCY-VIRUS TYPE-1 ; ENVELOPE GLYCOPROTEIN GP120 ; HAMSTER OVARY CELLS ; INACTIVATING PROTEIN ; V3 LOOP ; BINDING ; FUSION ; 2G12 ; AIDS ; CD4 |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000250679100016 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375347 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Shattock, Robin J. |
| 作者单位 | 1.St Georges Univ London, Ctr Infect, Dept Cellular & Mol Med, London SW17 0RE, England 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Qinxue,Mahmood, Naheed,Shattock, Robin J.. High-mannose-specific deglycosylation of hiv-1 gp120 induced by resistance to cyanovirin-n and the impact on antibody neutralization[J]. Virology,2007,368(1):145-154. |
| APA | Hu, Qinxue,Mahmood, Naheed,&Shattock, Robin J..(2007).High-mannose-specific deglycosylation of hiv-1 gp120 induced by resistance to cyanovirin-n and the impact on antibody neutralization.Virology,368(1),145-154. |
| MLA | Hu, Qinxue,et al."High-mannose-specific deglycosylation of hiv-1 gp120 induced by resistance to cyanovirin-n and the impact on antibody neutralization".Virology 368.1(2007):145-154. |
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来源：武汉病毒研究所
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