Loop-mediated isothermal amplification for rapid detection of bacillus anthracis spores
文献类型：期刊论文
| 作者 | Yan-Mei Qiao; Yong-Chao Guo; Xian-En Zhang; Ya-Feng Zhou; Zhi-Ping Zhang; Hong-Ping Wei; Rui-Fu Yang; Dian-Bing Wang |
| 刊名 | Biotechnology letters
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 29期号:12页码:1939-1946 |
| 关键词 | Bacillus anthracis Detection Isothermal amplification Lamp |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-007-9472-9 |
| 通讯作者 | Xian-en zhang(x.zhang@wh.iov.cn) |
| 英文摘要 | A loop-mediated isothermal amplification (lamp) assay system was employed for detecting bacillus anthracis spores in pure cultures as well as in various simulated powder samples. the specificity of the designed lamp primer sets was validated by assaying 13 b. anthracis strains and 33 non-b. anthracis species. the detection limits of the lamp assay were 10 spores/tube for pure cultures and 100 spores/2 mg powder for simulated powder samples. the results show that the lamp protocol is a promising method for detecting b. anthracis. |
| WOS关键词 | REAL-TIME PCR ; IDENTIFICATION ; SAMPLES ; ASSAY |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000250537700020 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375352 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Xian-En Zhang |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan-Mei Qiao,Yong-Chao Guo,Xian-En Zhang,et al. Loop-mediated isothermal amplification for rapid detection of bacillus anthracis spores[J]. Biotechnology letters,2007,29(12):1939-1946. |
| APA | Yan-Mei Qiao.,Yong-Chao Guo.,Xian-En Zhang.,Ya-Feng Zhou.,Zhi-Ping Zhang.,...&Dian-Bing Wang.(2007).Loop-mediated isothermal amplification for rapid detection of bacillus anthracis spores.Biotechnology letters,29(12),1939-1946. |
| MLA | Yan-Mei Qiao,et al."Loop-mediated isothermal amplification for rapid detection of bacillus anthracis spores".Biotechnology letters 29.12(2007):1939-1946. |
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来源：武汉病毒研究所
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