Characterization of a cryptic plasmid from bacillus sphaericus strain lp1-g
文献类型：期刊论文
| 作者 | Wu, Enying; Jun, Liang; Yuan Yongming; Yan Jianping; Berry, Colin; Yuan Zhiming |
| 刊名 | Plasmid
 |
| 出版日期 | 2007-05-01 |
| 卷号 | 57期号:3页码:296-305 |
| 关键词 | Bacillus sphaericus Cryptic plasmid Rolling-circle replication Replication initiator protein Ssdna |
| ISSN号 | 0147-619X |
| DOI | 10.1016/j.plasmid.2006.11.003 |
| 通讯作者 | Yuan zhiming(yzm@pentium.whiov.ac.cn) |
| 英文摘要 | A cryptic plasmid from bacillus sphaericus strain lp1-g, designated as plg, was sequenced and characterized. it was an 11,066 bp circular molecule, with g + c content of 37%. the plasmid plg was predicted to encode 23 putative orfs, and orf 21 shared the highest identity with rep of pg11 and pbmb9741, members of rolling-circle replication (rcr) pc194-family. sequence analysis revealed a pc194-type double strand origin (dso) and a single strand origin (sso) like sequence located upstream and downstream of orf 21, respectively. moreover, mung bean nuclease analysis and southern hybridization confirmed the existence of single stranded dna (ssdna) inter-mediates, indicating that plg belongs to the rcr pc194-family. accumulation of multiple ssdna intermediates in native strain lp1-g and decline of ssdna and supercoiled dna in rifampicin-treated strain implied that a special mechanism might be employed by plg. furthermore, the copy number of plg in its original host was determined and about 58 copies of the plasmid exist in each cell. subcloning and transformation experiments proved that the minimal replicon of plg was within a 1.6-kb fragment, which was composed of rep gene and dso. these data are a good basis for the understanding of replication mechanisms and genetics of this b. sphaericus plasmid. (c) 2006 elsevier inc. all rights reserved. |
| WOS关键词 | ROLLING-CIRCLE REPLICATION ; NUCLEOTIDE-SEQUENCE ; CULEX-QUINQUEFASCIATUS ; CONJUGATIVE PLASMID ; THURINGIENSIS ; GENE ; IDENTIFICATION ; TOXICITY ; TOXIN ; PREDICTION |
| WOS研究方向 | Genetics & Heredity |
| WOS类目 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000246566800005 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375356 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yuan Zhiming |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Cardiff Univ, Cardiff Sch Biosci, Cardiff CF10 3US, S Glam, Wales |
| 推荐引用方式 GB/T 7714 | Wu, Enying,Jun, Liang,Yuan Yongming,et al. Characterization of a cryptic plasmid from bacillus sphaericus strain lp1-g[J]. Plasmid,2007,57(3):296-305. |
| APA | Wu, Enying,Jun, Liang,Yuan Yongming,Yan Jianping,Berry, Colin,&Yuan Zhiming.(2007).Characterization of a cryptic plasmid from bacillus sphaericus strain lp1-g.Plasmid,57(3),296-305. |
| MLA | Wu, Enying,et al."Characterization of a cryptic plasmid from bacillus sphaericus strain lp1-g".Plasmid 57.3(2007):296-305. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。