A label-free colorimetric aptasensor for simple, sensitive and selective detection of pt (ii) based on platinum (ii)-oligonucleotide coordination induced gold nanoparticles aggregation
文献类型：期刊论文
| 作者 | Fan, Daoqing1,2; Zhai, Qingfeng1,2; Zhou, Weijun1,2; Zhu, Xiaoqing1,2; Wang, Erkang1,2; Dong, Shaojun1,2 |
| 刊名 | Biosensors & bioelectronics
 |
| 出版日期 | 2016-11-15 |
| 卷号 | 85页码:771-776 |
| 关键词 | Gold nanoparticles Platinum ion Colorimetric Aptamer sensor Streptavidin |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2016.05.080 |
| 通讯作者 | Dong, shaojun(dongsj@ciac.ac.cn) |
| 英文摘要 | Herein, a gold nanoparticles (aunps) based label-free colorimetric aptasensor for simple, sensitive and selective detection of pt (ii) was constructed for the first time. four bases (g-g mismatch) mismatched streptavidin aptamer (msaa) was used to protect aunps from salt-induced aggregation and recognize pt (ii) specifically. only in the presence of pt (ii), coordination occurs between g-g bases and pt (ii), leading to the activation of streptavidin aptamer. streptavidin coated magnetic beads (mbs) were used as separation agent to separate pt (ii)-coordinated msaa. the residual less amount of msaa could not efficiently protect aunps anymore and aggregation of aunps will produce a colorimetric product. with the addition of pt (ii), a pale purple-to-blue color variation could be observed by the naked eye. a detection limit of 150 nm and a linear range from 0.6 mu m to 12.5 mu m for pt (ii) could be achieved without any amplification. (c) 2016 elsevier b.v. all rights reserved. |
| WOS关键词 | HYBRIDIZATION CHAIN-REACTION ; LIVING CELLS ; LIQUID-CHROMATOGRAPHY ; QUADRUPLEX DNAZYME ; HUMAN SERUM ; DNA ; CISPLATIN ; AMPLIFICATION ; OXALIPLATIN ; MICRORNA |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000382410100103 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375360 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Dong, Shaojun |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Daoqing,Zhai, Qingfeng,Zhou, Weijun,et al. A label-free colorimetric aptasensor for simple, sensitive and selective detection of pt (ii) based on platinum (ii)-oligonucleotide coordination induced gold nanoparticles aggregation[J]. Biosensors & bioelectronics,2016,85:771-776. |
| APA | Fan, Daoqing,Zhai, Qingfeng,Zhou, Weijun,Zhu, Xiaoqing,Wang, Erkang,&Dong, Shaojun.(2016).A label-free colorimetric aptasensor for simple, sensitive and selective detection of pt (ii) based on platinum (ii)-oligonucleotide coordination induced gold nanoparticles aggregation.Biosensors & bioelectronics,85,771-776. |
| MLA | Fan, Daoqing,et al."A label-free colorimetric aptasensor for simple, sensitive and selective detection of pt (ii) based on platinum (ii)-oligonucleotide coordination induced gold nanoparticles aggregation".Biosensors & bioelectronics 85(2016):771-776. |
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来源：中国科学院大学
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