A new cry toxin with a unique two-component dependency from bacillus sphaericus
文献类型：期刊论文
| 作者 | Jones, Gareth W.; Nielsen-Leroux, Christina; Yang, Yankun; Yuan, Zhiming; Dumas, Vinicius Fiuza; Monnerat, Rose Gomes; Berry, Colin |
| 刊名 | Faseb journal
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 21期号:14页码:4112-4120 |
| 关键词 | Insecticidal toxin Toxin evolution Toxin interactions |
| ISSN号 | 0892-6638 |
| DOI | 10.1096/fj.07-8913com |
| 通讯作者 | Berry, colin(berry@cf.ac.uk) |
| 英文摘要 | Highly pathogenic strains of bacillus sphaericus produce the mosquitocidal bin proteins, but resistance to this toxin can be produced under laboratory and field conditions. analysis of strains able to overcome this resistance revealed the presence of a previously undescribed type of two-component toxin. one subunit, cry48aa1, is related to the 3-domain crystal toxins of bacillus thuringiensis. uniquely for this type of protein, insect toxicity is only achieved in the presence of a second, accessory protein, cry49aa1. this protein is itself related to both the binary toxin of b. sphaericus and to cry35 and cry36 of b. thuringiensis, none of which require interaction with cry48aa1-like proteins for their activity. the necessity for both cry48aa1 and cry49aa1 components for pathogenicity, therefore, indicates an unprecedented interaction to generate toxicity. despite high potency for purified cry48aa1/cry49aa1 proteins (lc50 for third instar culex quinquefasciatus larvae: 15.9 ng/ml and 6.3 ng/ml respectively), bacteria producing them show suboptimal mosquitocidal activity due to low-level cry48aa1 production. this new toxin combination may indicate a fortuitous combination of members of the gene families that encode 3-domain cry toxins and binary-like toxins, permitting the "mix-and-match" evolution of a new component in the mosquitocidal armoury. -jones, g. w., nielsen-leroux, c., yang, y., yuan, z., dumas, v. f., monnerat, r. g., colin berry, c. a new cry toxin with a unique two-component dependency from bacillus sphaericus. |
| WOS关键词 | THURINGIENSIS SUBSP ISRAELENSIS ; VIRGIFERA-VIRGIFERA LECONTE ; WESTERN CORN-ROOTWORM ; CULEX-PIPIENS DIPTERA ; MOSQUITOCIDAL TOXIN ; BINARY TOXIN ; LABORATORY CULTURE ; CROSS-RESISTANCE ; PLASMID TRANSFER ; CRYSTAL TOXIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000251283500036 |
| 出版者 | FEDERATION AMER SOC EXP BIOL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375361 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Berry, Colin |
| 作者单位 | 1.Cardiff Univ, Cardiff Sch Biosci, Cardiff CF10 3US, Wales 2.Inst Pasteur, Dept Microbiol, Paris, France 3.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China 4.Parque Estacao Biol, Embrapa Recursos Genet & Biotecnol, Brasilia, DF, Brazil |
| 推荐引用方式 GB/T 7714 | Jones, Gareth W.,Nielsen-Leroux, Christina,Yang, Yankun,et al. A new cry toxin with a unique two-component dependency from bacillus sphaericus[J]. Faseb journal,2007,21(14):4112-4120. |
| APA | Jones, Gareth W..,Nielsen-Leroux, Christina.,Yang, Yankun.,Yuan, Zhiming.,Dumas, Vinicius Fiuza.,...&Berry, Colin.(2007).A new cry toxin with a unique two-component dependency from bacillus sphaericus.Faseb journal,21(14),4112-4120. |
| MLA | Jones, Gareth W.,et al."A new cry toxin with a unique two-component dependency from bacillus sphaericus".Faseb journal 21.14(2007):4112-4120. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。