Immune responses against severe acute respiratory syndrome coronavirus induced by virus-like particles in mice
文献类型：期刊论文
| 作者 | Lu, Xinya; Chen, Yao; Bai, Bingke; Hu, Hui; Tao, Ling; Yang, Jihong; Chen, Jianjun; Chen, Ze; Hu, Zhihong; Wang, Hanzhong |
| 刊名 | Immunology
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 122期号:4页码:496-502 |
| 关键词 | Baculovirus expression vector system Immune response Severe acute respiratory syndrome Coronavirus Vaccine Virus-like particles |
| ISSN号 | 0019-2805 |
| 通讯作者 | Wang, hanzhong(wanghz@wh.iov.cn) |
| 英文摘要 | Virus-like particles (vlps) represent a promising vaccine against severe acute respiratory syndrome coronavirus (sars cov). in this study, recombinant baculovirus vacs and vacme were constructed to express the s protein and the m and e proteins of sars cov, respectively. electron microscope analysis demonstrated the formation of vlps in vacme and vacs coinfected insect cells. mice immunized four times with vlps developed high antibody titres against sars cov. in addition, vlps elicited cell-mediated immunity as demonstrated by enhanced interferon-gamma and interleukin-4 production. vlps also conferred protective immunity against the infection of spike protein pseudotyped murine leukaemia virus. our findings demonstrate that sars cov vlps are immunogenic and can elicit strong sars cov-specific humoral and cellular immune responses in mice. this is the first study describing the immunogenicity of sars cov vlps, providing valuable data for developing a protective vaccine against sars cov infection. |
| WOS关键词 | SARS-ASSOCIATED CORONAVIRUS ; RECEPTOR-BINDING DOMAIN ; SPIKE PROTEIN ; NEUTRALIZING ANTIBODIES ; AMINO-ACIDS ; S-PROTEIN ; VACCINE ; PROTECTION ; CHALLENGE ; PSEUDOPARTICLES |
| WOS研究方向 | Immunology |
| WOS类目 | Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000250761900005 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375364 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Hanzhong |
| 作者单位 | 1.Chinese Acad Sci, Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Hunzhong Normal Univ, Coll Life Sci, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Xinya,Chen, Yao,Bai, Bingke,et al. Immune responses against severe acute respiratory syndrome coronavirus induced by virus-like particles in mice[J]. Immunology,2007,122(4):496-502. |
| APA | Lu, Xinya.,Chen, Yao.,Bai, Bingke.,Hu, Hui.,Tao, Ling.,...&Wang, Hanzhong.(2007).Immune responses against severe acute respiratory syndrome coronavirus induced by virus-like particles in mice.Immunology,122(4),496-502. |
| MLA | Lu, Xinya,et al."Immune responses against severe acute respiratory syndrome coronavirus induced by virus-like particles in mice".Immunology 122.4(2007):496-502. |
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来源：武汉病毒研究所
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