Kinetics of plasmid transfer among bacillus cereus group strains within lepidopteran larvae
文献类型：期刊论文
| 作者 | Yuan, Y. M.; Hu, X. M.; Liu, H. Z.; Hansen, Bjarne Munk; Yan, J. P.; Yuan, Z. M. |
| 刊名 | Archives of microbiology
 |
| 出版日期 | 2007-06-01 |
| 卷号 | 187期号:6页码:425-431 |
| 关键词 | Bacillus thuringiensis Bacillus cereus Conjugation Insect larvae Plasmid |
| ISSN号 | 0302-8933 |
| DOI | 10.1007/s00203-006-0206-5 |
| 通讯作者 | Yuan, z. m.(yzm@pentium.whiov.ac.cn) |
| 英文摘要 | The cry toxin encoding plasmid pht73 was transferred from bacillus thuringiensis subspecies kurstaki kt0 to six b. cereus group strains in three lepidopteran ( spodoptera exigua, plutella xyllostella and helicoverpa armigera) larvae by conjugation. the conjugation kinetics of the plasmid was precisely studied during the larval infection using a new protocol. the infections were performed with both vegetative and sporulated strains. however, larval death only occurred when infections were made with spore and toxin preparations. likewise, spore germinations of both donor and recipient strains were only observed in killed larvae, 44-56 h post-infection. accordingly, kinetics showed that gene transfer between b. thuringiensis strain kt0 and other b. cereus strains only took place in dead larvae among vegetatively growing bacteria. the conjugational transfer ratios varied among different strain combinations and different larvae. the highest transfer ratio reached 5.83 x 10(-6) cfu/donor between the kt0 and the aw05r recipient in helicoverpa armigera, and all transconjugants gained the ability to produce the insecticidal crystal. these results indicated that horizontal gene transfer among b. cereus group strains might play a key role for the acquisition of extra plasmids and evolution of these strains in toxin susceptible insect larvae. |
| WOS关键词 | THURINGIENSIS SUBSP ISRAELENSIS ; CONJUGATIVE TRANSFER ; LABORATORY CULTURE ; AGGREGATION ; KURSTAKI ; SYSTEM ; EXPRESSION ; SURVIVAL ; PXO16 ; SOIL |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000246747300001 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375366 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yuan, Z. M. |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Natl Environm Res Inst, DK-4000 Roskilde, Denmark |
| 推荐引用方式 GB/T 7714 | Yuan, Y. M.,Hu, X. M.,Liu, H. Z.,et al. Kinetics of plasmid transfer among bacillus cereus group strains within lepidopteran larvae[J]. Archives of microbiology,2007,187(6):425-431. |
| APA | Yuan, Y. M.,Hu, X. M.,Liu, H. Z.,Hansen, Bjarne Munk,Yan, J. P.,&Yuan, Z. M..(2007).Kinetics of plasmid transfer among bacillus cereus group strains within lepidopteran larvae.Archives of microbiology,187(6),425-431. |
| MLA | Yuan, Y. M.,et al."Kinetics of plasmid transfer among bacillus cereus group strains within lepidopteran larvae".Archives of microbiology 187.6(2007):425-431. |
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来源：武汉病毒研究所
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