Comparison of immunogenicity between codon optimized hiv-1 thailand subtype b gp140 and gp145 vaccines
文献类型：期刊论文
| 作者 | Wan, Yanmin; Wu, Lan; Liu, Lianxing; Xu, Jianqing; Liu, Ying; Liu, Yong; Shao, Yiming |
| 刊名 | Vaccine
 |
| 出版日期 | 2007-06-21 |
| 卷号 | 25期号:26页码:4949-4959 |
| 关键词 | Hiv-1 Vaccine Envelope Immunogen design |
| ISSN号 | 0264-410X |
| DOI | 10.1016/j.vaccine.2007.01.118 |
| 通讯作者 | Shao, yiming(yshao@bbn.cn) |
| 英文摘要 | Hiv-1 pandemic posed an unprecedent challenge to the global health and it is believed that an effective vaccine will be the final solution against hiv-1. hiv-1 envelope is the primary immunogen in developing neutralization antibody based hiv vaccine. to define the suitable env derived immunogen, we systemically compared the immunogenicity of gp140 and gp145 in a dna vaccination alone and a prime-boost modalities. two dna vaccines and two recombinant tiantan vaccinia vaccines (rttv) were constructed for vaccination of female balb/c mice. elispot assay was used to read out the t cell immunity and elisa assay was used to quantify antibody immunity. pll (poly-l-leucine)elisa assay was used in linear antibody epitope mapping. mice primed with gp145 tended to elicit more env-specific t cells responses than those primed with gp140, significant difference was observed in dna immunization alone. the ultimate t cell responses in prime-boost regimen tend to be determined mainly by the priming efficacy. linear antibody epitope mapping displayed that sera raised by gp145 priming were vigorously reactive to more peptides than that by gp140. our data demonstrated hiv-1 thailand b-derived gp145 may raise higher t-cell responses and broader linear peptide-specific antibody responses than gp140 does. however, it remains to be determined that how these observations are relevant to the neutralization of antibody activities. (c) 2007 elsevier ltd. all rights reserved. |
| WOS关键词 | HUMAN-IMMUNODEFICIENCY-VIRUS ; TYPE-1 ENVELOPE GLYCOPROTEINS ; ELICIT NEUTRALIZING ANTIBODIES ; CANDIDATE AIDS VACCINES ; MONOCLONAL-ANTIBODY ; RECOMBINANT GP120 ; VARIABLE LOOPS ; TRIMERIC FORM ; RESPONSES ; IMMUNIZATION |
| WOS研究方向 | Immunology ; Research & Experimental Medicine |
| WOS类目 | Immunology ; Medicine, Research & Experimental |
| 语种 | 英语 |
| WOS记录号 | WOS:000247547400011 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375374 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Shao, Yiming |
| 作者单位 | 1.Chinese Ctr Dis Control & Prevent, Natl Ctr AIDS STD Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 100050, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China 3.Grad Univ, Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan, Yanmin,Wu, Lan,Liu, Lianxing,et al. Comparison of immunogenicity between codon optimized hiv-1 thailand subtype b gp140 and gp145 vaccines[J]. Vaccine,2007,25(26):4949-4959. |
| APA | Wan, Yanmin.,Wu, Lan.,Liu, Lianxing.,Xu, Jianqing.,Liu, Ying.,...&Shao, Yiming.(2007).Comparison of immunogenicity between codon optimized hiv-1 thailand subtype b gp140 and gp145 vaccines.Vaccine,25(26),4949-4959. |
| MLA | Wan, Yanmin,et al."Comparison of immunogenicity between codon optimized hiv-1 thailand subtype b gp140 and gp145 vaccines".Vaccine 25.26(2007):4949-4959. |
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来源：武汉病毒研究所
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