Development of a virus concentration method and its application for the detection of noroviruses in drinking water in china
文献类型：期刊论文
| 作者 | Liu, Junyi; Wu, Qingping; Kou, Xiaoxia |
| 刊名 | Journal of microbiology
 |
| 出版日期 | 2007-02-01 |
| 卷号 | 45期号:1页码:48-52 |
| 关键词 | Noroviruses Concentration Drinking water |
| ISSN号 | 1225-8873 |
| 通讯作者 | Kou, xiaoxia(mark771@163.com) |
| 英文摘要 | A new procedure for the concentration of nonoviruses from water samples has been developed. this procedure (calcium flocculation-citrate dissolution method) uses the following steps: virus flocculation formed by treatment with 1 m cacl2 and 1 m na2hpo4, virus release by sodium citrate dissolution (0.3 m na citrate, ph 3.5), and virus re-concentration by ultrafiltration. when reverse transcription (rt)-pcr was performed after the procedure, the overall detection sensitivity for seeded noroviruses in a one liter drinking water sample was as low as i rt-pcr unit, which is equal to a 10(-6) dilution of the virus sample. this approach showed at least a 5-fold-higher sensitivity than the current method with its three steps of adsorption-elution-concentration. the newly developed procedure was used to test different brands of bottled drinking water from china for putative contamination with noroviruses. a total of 144 samples were analyzed; all of the samples were negative for norovirus specific nucleic acids. |
| WOS关键词 | POLYMERASE CHAIN-REACTION ; ROUND-STRUCTURED VIRUSES ; ENTERIC VIRUSES ; MINERAL WATERS ; TAP WATER ; RT-PCR ; GASTROENTERITIS ; ENTEROVIRUSES ; GROUNDWATER ; POLIOVIRUS |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000244588800009 |
| 出版者 | MICROBIOLOGY SOC KOREA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375381 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Kou, Xiaoxia |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Guangdong Inst Microbiol, Guangdong Provincial Key Lab Microbial Culture Co, Guangzhou 510070, Peoples R China 3.Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Junyi,Wu, Qingping,Kou, Xiaoxia. Development of a virus concentration method and its application for the detection of noroviruses in drinking water in china[J]. Journal of microbiology,2007,45(1):48-52. |
| APA | Liu, Junyi,Wu, Qingping,&Kou, Xiaoxia.(2007).Development of a virus concentration method and its application for the detection of noroviruses in drinking water in china.Journal of microbiology,45(1),48-52. |
| MLA | Liu, Junyi,et al."Development of a virus concentration method and its application for the detection of noroviruses in drinking water in china".Journal of microbiology 45.1(2007):48-52. |
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来源：武汉病毒研究所
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