Baculovirus-mediated gene silencing in insect cells using intracellularly produced long double-stranded rna
文献类型：期刊论文
| 作者 | Huang, Yi; Deng, Fei; Hu, Zhihong; Vlak, Just M.; Wang, Hanzhong |
| 刊名 | Journal of biotechnology
 |
| 出版日期 | 2007-02-01 |
| 卷号 | 128期号:2页码:226-236 |
| 关键词 | Rnai Long hairpin dsrna Dna vectors Baculovirus Enhanced green fluorescent protein (egfp) Gene expression |
| ISSN号 | 0168-1656 |
| DOI | 10.1016/j.jbiotec.2006.09.016 |
| 通讯作者 | Wang, hanzhong(wanghz@pentium.whiov.ac.cn) |
| 英文摘要 | Double-stranded rna-mediated interference (rnai) has recently emerged as a powerful reverse genetics tool to silence gene expression in multiple organisms, including plants, nematodes and insects. in this study, dna vectors capable of promoting the synthesis of long hairpin dsrnas in vivo from a dna template to suppress gene expression in insect cells have been successfully constructed. the inhibition of the expression of a gene encoding enhanced green fluorescent protein (egfp) in insect cells was demonstrated by using plasmid or baculovirus vectors. both plasmid and baculovirus vectors were able to inhibit egfp expression in a dose dependent manner. complete inhibition was obtained when co-transfection ratios of target plasmid to inhibition plasmid were 1:1 and 1:0.1. eighty percent suppression was still maintained even when the ratio of egfp plasmid to 'hairpin' plasmid was as high as 1:0.01. when the hairpin dsrnas were encoded in a baculovirus, the suppression was about 50% when the ratio of 'target' baculovirus to 'inhibition' baculovirus reached 1:10. therefore, the designed plasmid and baculovirus vectors are useful to induce rnai in insect cell systems. (c) 2006 elsevier b.v. all rights reserved. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; CAENORHABDITIS-ELEGANS ; MESSENGER-RNA ; IN-VITRO ; INTERFERENCE ; EXPRESSION ; DROSOPHILA ; APOPTOSIS ; SUPPRESS ; POTENT |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000244026900002 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375383 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Hanzhong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Univ Wageningen & Res Ctr, Virol Lab, NL-6709 PD Wageningen, Netherlands 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Yi,Deng, Fei,Hu, Zhihong,et al. Baculovirus-mediated gene silencing in insect cells using intracellularly produced long double-stranded rna[J]. Journal of biotechnology,2007,128(2):226-236. |
| APA | Huang, Yi,Deng, Fei,Hu, Zhihong,Vlak, Just M.,&Wang, Hanzhong.(2007).Baculovirus-mediated gene silencing in insect cells using intracellularly produced long double-stranded rna.Journal of biotechnology,128(2),226-236. |
| MLA | Huang, Yi,et al."Baculovirus-mediated gene silencing in insect cells using intracellularly produced long double-stranded rna".Journal of biotechnology 128.2(2007):226-236. |
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来源：武汉病毒研究所
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