Molecular cloning and heterologous expression of a new xylanase gene from plectosphaerella cucumerina
文献类型：期刊论文
| 作者 | Zhang, Gui Min; Huang, Jun; Huang, Guang Rui; Ma, Li Xin; Zhang, Xian En |
| 刊名 | Applied microbiology and biotechnology
 |
| 出版日期 | 2007-02-01 |
| 卷号 | 74期号:2页码:339-346 |
| 关键词 | Xylanase Plectosphaerella cucumerina Genome-walking pcr Heterologous expression Pichia pastoris |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-006-0648-3 |
| 通讯作者 | Ma, li xin(malixing@hubu.edu.cn) |
| 英文摘要 | A gene encoding a new xylanase, named xynzg, was cloned by the genome-walking pcr method from the nematophagous fungus plectosphaerella cucumerina. the genomic dna sequence of xynzg contains a 780 bp open reading frame separated by two introns with the sizes of 50 and 46 bp. to our knowledge, this would be the first functional gene cloned from p. cucumerina. the 684 bp cdna was cloned into vector phbm905b and transformed into pichia pastoris gs115 to select xylanase-secreting transformants on rbb-xylan containing plate. the optimal secreting time was 3 days at 25 degrees c and enzymatic activities in the culture supernatants reached the maximum level of 362 u ml(.)(-1) the molecular mass of the enzyme was estimated to be 19 kda on sds-page. the optimal ph and temperature of the purified enzyme is 6 and 40 degrees c, respectively. the purified enzyme is stable at room temperature for at least 10 h. the k-m and v-max values for birchwood xylan are 2.06 mg ml(-1) and 0.49 mmol min(-1) mg(-1), respectively. the inhibitory effects of various mental ions were investigated. it is interesting to note that cu2+ ion, which strongly inhibits most other xylanases studied, reduces enzyme activity by only 40%. furthermore, enzyme activity is unaffected by edta even at a concentration of 5 mm. |
| WOS关键词 | ASPERGILLUS-NIGER ; KRAFT PULP ; PURIFICATION ; ENZYMES ; STRAIN |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000243961500011 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375390 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Ma, Li Xin |
| 作者单位 | 1.Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 3.Huazhong Agr Univ, State Key Lab Agr Microorganism, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Gui Min,Huang, Jun,Huang, Guang Rui,et al. Molecular cloning and heterologous expression of a new xylanase gene from plectosphaerella cucumerina[J]. Applied microbiology and biotechnology,2007,74(2):339-346. |
| APA | Zhang, Gui Min,Huang, Jun,Huang, Guang Rui,Ma, Li Xin,&Zhang, Xian En.(2007).Molecular cloning and heterologous expression of a new xylanase gene from plectosphaerella cucumerina.Applied microbiology and biotechnology,74(2),339-346. |
| MLA | Zhang, Gui Min,et al."Molecular cloning and heterologous expression of a new xylanase gene from plectosphaerella cucumerina".Applied microbiology and biotechnology 74.2(2007):339-346. |
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来源：武汉病毒研究所
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