An intrastrain variation of a 189-bp repeat region in the human cytomegalovirus replication origin
文献类型：期刊论文
| 作者 | Chen, Z.; Watanabe, S. |
| 刊名 | Acta virologica
 |
| 出版日期 | 2007 |
| 卷号 | 51期号:1页码:21-26 |
| 关键词 | Human cytomegalovirus Dna replication Replication origin |
| ISSN号 | 0001-723X |
| 通讯作者 | Chen, z.(chenze2005@263.net) |
| 英文摘要 | A 189-bp repeat has been reported previously to be present in the replication origin (ori) of human cytomegalovirus (hcmv) towne strain and to contain essential and supporting sequences (chen et al., arch. virol. 141, 13-301 (1996)). in this study, clones of hcmv strain towne were isolated and analyzed for the copy number of the 189-bp repeat within its ori (orilyt). southern blot analysis revealed that out of 14 clones, two contained a dimer, two contained a tetramer, and the remaining contained a trimer of the 189-bp repeat. nucleotide sequence analysis disclosed that, within the dimer and the tetramer, the 189-bp repeat was present in a directly repeated manner. in this way we demonstrated an hcmv intrastrain variation in the copy number of the 189-bp repeat. dot blot hybridization and growth curve analysis showed that the clones containing the dimer or the tetramer replicated in infected cells with similar efficiencies. we conclude that the hcmv orilyt with spatial separation of the essential flanking regions by various copy number of the 189-bp repeat acts as ori in the viral genome. |
| WOS关键词 | SIMPLEX-VIRUS TYPE-1 ; DEPENDENT DNA-REPLICATION ; INFECTED PATIENTS ; ORILYT ; SEQUENCE ; PROTEIN ; CLONING ; HIV ; IDENTIFICATION ; TRANSCRIPTION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000246098700003 |
| 出版者 | SLOVAK ACADEMIC PRESS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375392 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Z. |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Natl Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China 3.Univ Tokyo, Inst Med Sci, Dept Virol, Tokyo 108, Japan |
| 推荐引用方式 GB/T 7714 | Chen, Z.,Watanabe, S.. An intrastrain variation of a 189-bp repeat region in the human cytomegalovirus replication origin[J]. Acta virologica,2007,51(1):21-26. |
| APA | Chen, Z.,&Watanabe, S..(2007).An intrastrain variation of a 189-bp repeat region in the human cytomegalovirus replication origin.Acta virologica,51(1),21-26. |
| MLA | Chen, Z.,et al."An intrastrain variation of a 189-bp repeat region in the human cytomegalovirus replication origin".Acta virologica 51.1(2007):21-26. |
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来源：武汉病毒研究所
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