Kaposi's sarcoma-associated herpesvirus infection promotes invasion of primary human umbilical vein endothelial cells by inducing matrix metalloproteinasesv
文献类型：期刊论文
| 作者 | Qian, Li-Wu; Xie, Hanping; Ye, Fengchun; Gao, Shou-Jiang |
| 刊名 | Journal of virology
 |
| 出版日期 | 2007-07-01 |
| 卷号 | 81期号:13页码:7001-7010 |
| ISSN号 | 0022-538X |
| DOI | 10.1128/jvi.00016-07 |
| 通讯作者 | Gao, shou-jiang(gaos@uthscsa.edu) |
| 英文摘要 | Matrix metalloproteinases (mmps) play important roles in cancer invasion, angiogenesis, and inflammatory infiltration. kaposi's sarcoma is a highly disseminated angiogenic tumor of proliferative endothelial cells linked to infection by kaposi's sarcoma-associated herpesvirus (kshv). in this study, we showed that kshv infection increased the invasiveness of primary human umbilical vein endothelial cells (huvec) in a matrigel-based cell invasion assay. kshv-induced cell invasion was abolished by an inhibitor of mmps, bb-94, and occurred in both autocrine- and paracrine-dependent fashions. analysis by zymography and western blotting showed that kshv-infected huvec cultures had increased secretion of mmp-1, -2, and -9. kshv increased the secretion of mmp-2 within 1 h following infection without upregulating its mrna expression level. in contrast, the secretion of mmp-1 and -9 was not increased until 6 h after kshv infection and was correlated with the upregulation of their mrna expression levels. promoter analysis by reporter assays and electrophoretic mobility shift assays identified an ap-1 cis-element as the dominant kshv-responsive site in the mmp-1 promoter. together, these results suggest that kshv infection modulates the production of multiple mmps to increase cell invasiveness and thus contributes to the pathogenesis of kshv-induced malignancies. |
| WOS关键词 | ACTIVATED PROTEIN-KINASE ; NF-KAPPA-B ; SIGNALING PATHWAY ; MEMBRANE-PROTEIN ; GENE INDUCTION ; EXPRESSION ; CANCER ; ANGIOGENESIS ; INHIBITORS ; FIBROBLAST |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000247404900023 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375397 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Gao, Shou-Jiang |
| 作者单位 | 1.Univ Texas, Hlth Sci Ctr, Tumor Virol Program, Greehey Childrens Canc Res Inst, San Antonio, TX 78229 USA 2.Univ Texas, Hlth Sci Ctr, Dept Pediat, San Antonio, TX 78229 USA 3.Univ Texas, Hlth Sci Ctr, Dept Microbiol & Immunol, San Antonio, TX 78229 USA 4.Univ Texas, Hlth Sci Ctr, Dept Mol Med, San Antonio, TX 78229 USA 5.Univ Texas, Hlth Sci Ctr, San Antonio Canc Inst, San Antonio, TX 78229 USA 6.Chinese Acad Sci, Wuhan Inst Virol, Tumor Virol Grp, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qian, Li-Wu,Xie, Hanping,Ye, Fengchun,et al. Kaposi's sarcoma-associated herpesvirus infection promotes invasion of primary human umbilical vein endothelial cells by inducing matrix metalloproteinasesv[J]. Journal of virology,2007,81(13):7001-7010. |
| APA | Qian, Li-Wu,Xie, Hanping,Ye, Fengchun,&Gao, Shou-Jiang.(2007).Kaposi's sarcoma-associated herpesvirus infection promotes invasion of primary human umbilical vein endothelial cells by inducing matrix metalloproteinasesv.Journal of virology,81(13),7001-7010. |
| MLA | Qian, Li-Wu,et al."Kaposi's sarcoma-associated herpesvirus infection promotes invasion of primary human umbilical vein endothelial cells by inducing matrix metalloproteinasesv".Journal of virology 81.13(2007):7001-7010. |
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来源：武汉病毒研究所
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