Molecular characterization of a glucokinase with broad hexose specificity from bacillus sphaericus strain c3-41
文献类型：期刊论文
| 作者 | Han, Bei; Liu, Haizhou; Hu, Xiaomin; Cai, Yajun; Zheng, Dasheng; Yuan, Zhiming |
| 刊名 | Applied and environmental microbiology
 |
| 出版日期 | 2007-06-01 |
| 卷号 | 73期号:11页码:3581-3586 |
| ISSN号 | 0099-2240 |
| DOI | 10.1128/aem.02863-06 |
| 通讯作者 | Yuan, zhiming(yzm@pentium.whiov.ac.cn) |
| 英文摘要 | Bacillus sphaericus cannot metabolize sugar since it lacks several of the enzymes necessary for glycolysis. our results confirmed the presence of a glucokinase-encoding gene, glck, and a phosphofructokinase-encoding gene, pfk, on the bacterial chromosome and expression of glucokinase during vegetative growth of b. sphaericus strains. however, no phosphoglucose isomerase gene (pgi) or phosphoglucose isomerase enzyme activity was detected in these strains. furthermore, one glck open reading frame was cloned from b. sphaericus strain c3-41 and then expressed in escherichia coli. biochemical analysis revealed that this gene encoded a protein with a molecular mass of 33 kda and that the purified recombinant glucolkinase had k-m values of 0.52 and 0.31 mm for atp and glucose, respectively. it has been proved that this atp-dependent glucokinase can also phosphorylate fructose and mannose, and sequence alignment of the glck gene indicated that it belongs to the rok protein family. it is postulated that the absence of the phosphoglucose isomerase-encoding gene pgi in b. sphaericus might be one of the reasons for the inability of this bacterium to metabolize carbohydrates. our findings provide additional data that further elucidate the specific metabolic pathway and could be used for genetic improvement of b. sphaericus. |
| WOS关键词 | GLUCOSE KINASE ; DEPENDENT GLUCOKINASE ; PHOSPHORYLATION ; IDENTIFICATION ; HEXOKINASES ; METABOLISM ; SUBTILIS ; PROTEIN ; SYSTEM ; SPORES |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000247016600016 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375400 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yuan, Zhiming |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Bei,Liu, Haizhou,Hu, Xiaomin,et al. Molecular characterization of a glucokinase with broad hexose specificity from bacillus sphaericus strain c3-41[J]. Applied and environmental microbiology,2007,73(11):3581-3586. |
| APA | Han, Bei,Liu, Haizhou,Hu, Xiaomin,Cai, Yajun,Zheng, Dasheng,&Yuan, Zhiming.(2007).Molecular characterization of a glucokinase with broad hexose specificity from bacillus sphaericus strain c3-41.Applied and environmental microbiology,73(11),3581-3586. |
| MLA | Han, Bei,et al."Molecular characterization of a glucokinase with broad hexose specificity from bacillus sphaericus strain c3-41".Applied and environmental microbiology 73.11(2007):3581-3586. |
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来源：武汉病毒研究所
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