Deletion of a helicoverpa armigera nucleopolyhedrovirus gene encoding a virion structural protein (orf 107) increases the budded virion titre and reduces in vivo infectivity
文献类型：期刊论文
| 作者 | Pan, Xiaoyu1,2; Long, Gang1,3; Wang, RanRan1,2; Hou, Songwang1; Wang, Huiyuan1,2; Zheng, Yuetinq1; Sun, Xiulian1; Westenberg, Marcel3; Deng, Fei1; Wang, Hualin1 |
| 刊名 | Journal of general virology
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 88页码:3307-3316 |
| ISSN号 | 0022-1317 |
| DOI | 10.1099/vir-0.83363-0 |
| 通讯作者 | Hu, zhihong(huzh@wh.iov.cn) |
| 英文摘要 | The open reading frame ha107 of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (hearnpv) encodes a putative protein of 51 kda with homologues in a few group 11 npvs and a granulovirus. ha107 was transcribed as polyadenylated transcripts in infected hzam1 insect cells. the transcripts were initiated at two distinct locations, one upstream of ha 106 (superoxide dismutase gene, sod) and the second upstream of ha 107. by western blot analysis, two forms of the ha 107 protein were detected in infected cells, a major polypeptide of 48 kda and a minor one of 51 kda. western blot and immunoelectron microscopy analyses further showed that the ha 107 protein was associated with the nucleocapsids of both budded virions (bvs) and occlusion-derived virions. a ha107 knockout virus expressing enhanced green fluorescent protein and polyhedrin was constructed using bacmid technology. a one-step virus growth curve indicated that the bv titre of the knockout virus was significantly higher than that of the parental virus and a ha107 repair virus. bioassays indicated that the knockout virus was able to infect third-instar h. armigera larvae; however, its median lethal dose (ld50) was significantly higher than those of the parental virus and ha 107 repair virus. these data indicate that ha 107 encodes a non-essential structural protein of hearnpv virions and that deletion of this gene increases the bv titre and ld50 of the occluded virus. |
| WOS关键词 | SINGLE-NUCLEOCAPSID NUCLEOPOLYHEDROVIRUS ; ESCHERICHIA-COLI ; GENOME SEQUENCE ; BACULOVIRUSES ; CLONING ; VIRUS ; DNA |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000251619500013 |
| 出版者 | SOC GENERAL MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375405 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Hu, Zhihong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Univ Wageningen & Res Ctr, Virol Lab, NL-6709 PD Wageningen, Netherlands |
| 推荐引用方式 GB/T 7714 | Pan, Xiaoyu,Long, Gang,Wang, RanRan,et al. Deletion of a helicoverpa armigera nucleopolyhedrovirus gene encoding a virion structural protein (orf 107) increases the budded virion titre and reduces in vivo infectivity[J]. Journal of general virology,2007,88:3307-3316. |
| APA | Pan, Xiaoyu.,Long, Gang.,Wang, RanRan.,Hou, Songwang.,Wang, Huiyuan.,...&Hu, Zhihong.(2007).Deletion of a helicoverpa armigera nucleopolyhedrovirus gene encoding a virion structural protein (orf 107) increases the budded virion titre and reduces in vivo infectivity.Journal of general virology,88,3307-3316. |
| MLA | Pan, Xiaoyu,et al."Deletion of a helicoverpa armigera nucleopolyhedrovirus gene encoding a virion structural protein (orf 107) increases the budded virion titre and reduces in vivo infectivity".Journal of general virology 88(2007):3307-3316. |
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来源：武汉病毒研究所
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