Proteomics analysis of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus identified two new occlusion-derived virus-associated proteins, ha44 and ha100
文献类型：期刊论文
| 作者 | Deng, Fei; Wang, Ranran; Fang, Minggang; Jiang, Yue; Xu, Xushi; Wang, Hanzhong; Chen, Xinwen; Arif, Basil M.; Guo, Lin; Wang, Hualin |
| 刊名 | Journal of virology
 |
| 出版日期 | 2007-09-01 |
| 卷号 | 81期号:17页码:9377-9385 |
| ISSN号 | 0022-538X |
| DOI | 10.1128/jvi.00632-07 |
| 通讯作者 | Hu, zhihong(huzh@wh.iov.cn) |
| 英文摘要 | Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry were used to analyze the structural proteins of the occlusion-derived virus (odv) of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (hearnpv), a group ii npv. twenty-three structural proteins of hearnpv odv were identified, 21 of which have been reported previously as structural proteins or odv-associated proteins in other baculoviruses. these include polyhedrin, p78/83, p49, odv-e18, odv-ec27, odv-e56, p74, lef-3, ha66 (ac66), dna polymerase, gp41, vp39, p33, odv-e25, helicase, p6.9, odv/bv-c42, vp80, odv-ec43, odv-e66, and pif-1. two proteins encoded by hearnpv orf44 (ha44) and orf100 (ha100) were discovered as odv-associated proteins for the first time. ha44 encodes a protein of 378 aa with a predicted mass of 42.8 kda. ha100 encodes a protein of 510 aa with a predicted mass of 58.1 kda and is a homologue of the gene for poly (adp- ribose) glycohydrolase (parg). western blot analysis and immunoelectron microscopy confirmed that ra44 is associated with the nucleocapsid and ha100 is associated with both the nucleocapsid and the envelope of hearnpv odv. ha44 is conserved in group h npvs and granuloviruses but does not exist in group i npvs, while ha100 is conserved only in group ii npvs. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; AUTOGRAPHA-CALIFORNICA NUCLEOPOLYHEDROVIRUS ; ENVELOPE FUSION PROTEIN ; MULTIPLE SEQUENCE ALIGNMENT ; PER-OS-INFECTIVITY ; OPEN READING FRAME ; STRUCTURAL PROTEIN ; POLY(ADP-RIBOSE) GLYCOHYDROLASE ; TRANSCRIPTIONAL ANALYSIS ; NUCLEOTIDE-SEQUENCE |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000248923700050 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375407 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Hu, Zhihong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China 3.Great Lakes Forestry Ctr, Mol Virol Lab, Sault Ste Marie, ON, Canada 4.Wuhan Univ, State Key Lab Virol, Wuhan 430072, Peoples R China 5.Coll Life Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Fei,Wang, Ranran,Fang, Minggang,et al. Proteomics analysis of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus identified two new occlusion-derived virus-associated proteins, ha44 and ha100[J]. Journal of virology,2007,81(17):9377-9385. |
| APA | Deng, Fei.,Wang, Ranran.,Fang, Minggang.,Jiang, Yue.,Xu, Xushi.,...&Hu, Zhihong.(2007).Proteomics analysis of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus identified two new occlusion-derived virus-associated proteins, ha44 and ha100.Journal of virology,81(17),9377-9385. |
| MLA | Deng, Fei,et al."Proteomics analysis of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus identified two new occlusion-derived virus-associated proteins, ha44 and ha100".Journal of virology 81.17(2007):9377-9385. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。