Immortalizaton of swine umbilical vein endothelial cells with human telomerase reverse transcriptase
文献类型：期刊论文
| 作者 | Hong, Hai Xia1; Zhang, Yan Ming1; Xu, Hao1; Su, Zheng Yuan2; Sun, Pei1 |
| 刊名 | Molecules and cells
 |
| 出版日期 | 2007-12-31 |
| 卷号 | 24期号:3页码:358-363 |
| 关键词 | Human telomerase reverse transcriptase (htert) Immortalization Liposome Positive clone Swine umbilical vein endothelial cells (suvecs) Transfection |
| ISSN号 | 1016-8478 |
| 通讯作者 | Zhang, yan ming(ylzhangym@sohu.com) |
| 英文摘要 | Swine endothelial cells are commonly used as an in vitro model for studying features of the blood-brain barrier and some hemorrhagic diseases. however, primary cultures of swine cells have finite lifespans. to establish immortalized swine umbilical vein endothelial cells (suvecs) using human telomerase reverse transcriptase (htert), the plasmid pci-neo-htert was transfected into suvecs by lipofection. clones were selected for 6418 resistance, and positive clones were amplified. one of the clones was cultured for up to 50 passages. factor viii-related antigen and cd34 were detected. the immortalized cells shared the properties of normal cells, such as contact inhibition, serum requirement and anchorage dependence. karyotype analysis revealed that the immortalized cells were in the diploid range. in addition, both in vivo and in vitro assays of tumorigenicity showed no neoplastic transformation. furthermore, no, pgi(2), and et-1 concentrations in the transfected cells were normal. these results suggest that the suvecs immortalized by htert retain their original characteristics. |
| WOS关键词 | IN-VITRO ; CATALYTIC SUBUNIT ; HTERT GENE ; T-ANTIGEN ; EXPRESSION ; TRANSFECTION ; FIBROBLASTS ; SENESCENCE ; EXTENSION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000252248000008 |
| 出版者 | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375410 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Yan Ming |
| 作者单位 | 1.NW A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Dept Mol Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hong, Hai Xia,Zhang, Yan Ming,Xu, Hao,et al. Immortalizaton of swine umbilical vein endothelial cells with human telomerase reverse transcriptase[J]. Molecules and cells,2007,24(3):358-363. |
| APA | Hong, Hai Xia,Zhang, Yan Ming,Xu, Hao,Su, Zheng Yuan,&Sun, Pei.(2007).Immortalizaton of swine umbilical vein endothelial cells with human telomerase reverse transcriptase.Molecules and cells,24(3),358-363. |
| MLA | Hong, Hai Xia,et al."Immortalizaton of swine umbilical vein endothelial cells with human telomerase reverse transcriptase".Molecules and cells 24.3(2007):358-363. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。