Induction of specific immune responses by severe acute respiratory syndrome coronavirus spike dna vaccine with or without interleukin-2 immunization using different vaccination routes in mice
文献类型：期刊论文
| 作者 | Hu, Hui; Lu, Xinya; Tao, Ling; Bai, Bingke; Zhang, Zhenfeng; Chen, Yao; Zheng, Fangliang; Chen, Jianjun; Chen, Ze; Wang, Hanzhong |
| 刊名 | Clinical and vaccine immunology
 |
| 出版日期 | 2007-07-01 |
| 卷号 | 14期号:7页码:894-901 |
| ISSN号 | 1556-6811 |
| DOI | 10.1128/cvi.00019-07 |
| 通讯作者 | Wang, hanzhong(wanghz@wh.iov.cn) |
| 英文摘要 | Dna vaccines induce immoral and cellular immune responses in animal models and humans. to analyze the immunogenicity of the severe acute respiratory syndrome (sars) coronavirus (cov), sars-cov, spike dna vaccine and the immunoregulatory activity of interleukin-2 (il-2), dna vaccine plasmids pcdna-s and pcdna-il-2 were constructed and inoculated into balb/c mice with or without pcdna-il-2 by using three different immunization routes (the intramuscular route, electroporation, or the oral route with live attenuated salmonella enterica serovar typhimurium). the cellular and humoral immune responses were assessed by enzyme-linked immunosorbent assays, lymphocyte proliferation assays, enzyme-linked immunospot assays, and fluorescence-activated cell sorter analyses. the results showed that specific immoral and cellular immunities could be induced in mice by inoculating them with sars-cov spike dna vaccine alone or by coinoculation with il-2-expressing plasmids. in addition, the immune response levels in the coinoculation groups were significantly higher than those in groups receiving the spike dna vaccine alone. the comparison between the three vaccination routes indicated that oral vaccination evoked a vigorous t-cell response and a weak response predominantly with subclass immunoglobulin g2a (igg2a) antibody. however, intramuscular immunization evoked a vigorous antibody response and a weak t-cell response, and vaccination by electroporation evoked a vigorous response with a predominant subclass igg1 antibody response and a moderate t-cell response. our findings show that the spike dna vaccine has good immunogenicity and can induce specific humoral and cellular immunities in balb/c mice, while il-2 plays an immunoadjuvant role and enhances the humoral and cellular immune responses. different vaccination routes also evoke distinct immune responses. this study provides basic information for the design of dna vaccines against sars-cov. |
| WOS关键词 | HEPATITIS-B-VIRUS ; ATTENUATED SALMONELLA-TYPHIMURIUM ; TRANSMISSIBLE GASTROENTERITIS CORONAVIRUS ; SARS CORONAVIRUS ; NEUTRALIZING ANTIBODIES ; GENE-TRANSFER ; S-PROTEIN ; CELL ; MODEL ; ANTIGEN |
| WOS研究方向 | Immunology ; Infectious Diseases ; Microbiology |
| WOS类目 | Immunology ; Infectious Diseases ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000248074800012 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375414 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Hanzhong |
| 作者单位 | 1.Chinese Acad Sci, Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Hui,Lu, Xinya,Tao, Ling,et al. Induction of specific immune responses by severe acute respiratory syndrome coronavirus spike dna vaccine with or without interleukin-2 immunization using different vaccination routes in mice[J]. Clinical and vaccine immunology,2007,14(7):894-901. |
| APA | Hu, Hui.,Lu, Xinya.,Tao, Ling.,Bai, Bingke.,Zhang, Zhenfeng.,...&Wang, Hanzhong.(2007).Induction of specific immune responses by severe acute respiratory syndrome coronavirus spike dna vaccine with or without interleukin-2 immunization using different vaccination routes in mice.Clinical and vaccine immunology,14(7),894-901. |
| MLA | Hu, Hui,et al."Induction of specific immune responses by severe acute respiratory syndrome coronavirus spike dna vaccine with or without interleukin-2 immunization using different vaccination routes in mice".Clinical and vaccine immunology 14.7(2007):894-901. |
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来源：武汉病毒研究所
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