Construction and characterization of a helicoverpa armigera nucleopolyhedrovirus bacterial artificial chromosome with deletion of ecdysteroid udp-glucosyltransferase gene
文献类型：期刊论文
| 作者 | Si, Yan-Hong; Fang, Ming-Gang; Huang, Yi; Zheng, Fang-Liang; Li, Ting; Hu, Zhi-Hong; Wang, Han-Zhong |
| 刊名 | Bioscience biotechnology and biochemistry
 |
| 出版日期 | 2007-10-01 |
| 卷号 | 71期号:10页码:2435-2441 |
| 关键词 | Helicoverpa armigera nucleopolyhedrovirus (hearnpv) Bacmid Biological activity Ecdysteroid udp-glucosyltransferase gene |
| ISSN号 | 0916-8451 |
| DOI | 10.1271/bbb.70200 |
| 通讯作者 | Wang, han-zhong(wanghz@wh.iov.cn) |
| 英文摘要 | Genetically modified baculoviruses offer a promising alternative to chemical insecticides in the control of agricultural and forest insect pests. a novel bacmid, habacyh6, was constructed in which the eedysteroid udp-glucosyltransferase gene (egt) was replaced with a bacterial replication cassette containing a mini-f replicon, a kanamycin resistance gene, and the atttn7 site. insertion of the enhanced green fluorescence protein gene (egfp) into habacyh6 showed that the bacmid can express an active exogenous protein. bioassays showed that median lethal time (lt50) of habacyh6 was 89.23 h in third instar helicoverpa armigera larvae, 15.81 h earlier than that of wild-type hearnpv-g4, though there was no significant difference in median lethal dose (ld50). the data indicate that habacyh6 can be used as a new bac-to-bac system, and can also provide a technology platform for generating more effective biological insecticides. |
| WOS关键词 | SINGLE-NUCLEOCAPSID NUCLEOPOLYHEDROVIRUS ; NUCLEAR POLYHEDROSIS-VIRUS ; ESCHERICHIA-COLI ; DNA-REPLICATION ; RECOMBINANT BACULOVIRUSES ; INSECT CONTROL ; PROTEIN ; LEPIDOPTERA ; EXPRESSION ; PESTICIDE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry, Applied ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000250669000011 |
| 出版者 | TAYLOR & FRANCIS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375420 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Han-Zhong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Univ Georgia, Dept Pharmaceut & Biomed Sci, Coll Pharm, Athens, GA 30602 USA |
| 推荐引用方式 GB/T 7714 | Si, Yan-Hong,Fang, Ming-Gang,Huang, Yi,et al. Construction and characterization of a helicoverpa armigera nucleopolyhedrovirus bacterial artificial chromosome with deletion of ecdysteroid udp-glucosyltransferase gene[J]. Bioscience biotechnology and biochemistry,2007,71(10):2435-2441. |
| APA | Si, Yan-Hong.,Fang, Ming-Gang.,Huang, Yi.,Zheng, Fang-Liang.,Li, Ting.,...&Wang, Han-Zhong.(2007).Construction and characterization of a helicoverpa armigera nucleopolyhedrovirus bacterial artificial chromosome with deletion of ecdysteroid udp-glucosyltransferase gene.Bioscience biotechnology and biochemistry,71(10),2435-2441. |
| MLA | Si, Yan-Hong,et al."Construction and characterization of a helicoverpa armigera nucleopolyhedrovirus bacterial artificial chromosome with deletion of ecdysteroid udp-glucosyltransferase gene".Bioscience biotechnology and biochemistry 71.10(2007):2435-2441. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。