Live cell imaging of protein interactions in poliovirus rna replication complex using fluorescence resonance energy transfer (fret)
文献类型：期刊论文
| 作者 | Li, Na1,2; Cui, Zong-Qiang1; Wen, Ji-Kai1; Zhang, Zhi-Ping1; Wei, Hong-Ping1; Zhou, Ya-Feng1; Zhang, Xian-En1 |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2008-04-11 |
| 卷号 | 368期号:3页码:489-494 |
| ISSN号 | 0006-291X |
| 关键词 | Protein interactions Fret Poliovirus rna replication complex Live cells |
| DOI | 10.1016/j.bbrc.2008.01.094 |
| 通讯作者 | Zhang, xian-en(x.zhang@wh.iov.cn) |
| 英文摘要 | Poliovirus rna replication is directed by a replication complex on the rosette-like arrangement of membranous vesicles. proteins derived from the p3 region of the polioviral genome, such as 3d, 3ab, and 313 (vpg), play key roles in the formation and function of the replication complex. in the present study, by using an acceptor photobleaching protocol for fluorescence resonance energy transfer (fret) imaging, we visualized the interactions of 3d, 3ab, and vpg in living cells. the interaction of 3ab-vpg was determined by live cell fret analysis. quantitative analyses showed that the fret efficiencies of 3ab-3d, vpg-3d, and 3ab-vpg were 3.9 +/- 0.4% (n = 36), 4.5 +/- 0.4% (n = 39), and 8.3 +/- 0.6% (n = 44), respectively, in the cell cytoplasm where viral replication complexes are formed and function. poliovirus infection enhanced the protein interactions of vpg-3d and 3ab-3d, with fret efficiencies in the virus-infected cells of 10.7 +/- 1.1% (n = 39) and 9.0 +/- 7l 0.9% (n = 37), respectively. this method of live cell analysis of protein interactions in the poliovirus rna replication complex lays the foundation for further understanding of the real-time process of poliovirus rna replication. (c) 2008 elsevier inc. all rights reserved. |
| WOS关键词 | 3AB PROTEIN ; POLYMERASE ; REQUIRES ; VPG ; MICROSCOPY ; INITIATION ; VARIANTS ; 3D(POL) |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| WOS记录号 | WOS:000253925900007 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375425 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Xian-En |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Na,Cui, Zong-Qiang,Wen, Ji-Kai,et al. Live cell imaging of protein interactions in poliovirus rna replication complex using fluorescence resonance energy transfer (fret)[J]. Biochemical and biophysical research communications,2008,368(3):489-494. |
| APA | Li, Na.,Cui, Zong-Qiang.,Wen, Ji-Kai.,Zhang, Zhi-Ping.,Wei, Hong-Ping.,...&Zhang, Xian-En.(2008).Live cell imaging of protein interactions in poliovirus rna replication complex using fluorescence resonance energy transfer (fret).Biochemical and biophysical research communications,368(3),489-494. |
| MLA | Li, Na,et al."Live cell imaging of protein interactions in poliovirus rna replication complex using fluorescence resonance energy transfer (fret)".Biochemical and biophysical research communications 368.3(2008):489-494. |
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来源：武汉病毒研究所
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