Cobalt oxyhydroxide nanoflake based fluorescence sensing platform for label-free detection of dna
文献类型：期刊论文
| 作者 | Chang, Yaqing1,2; Zhang, Zhe1; Liu, Huiqing1,2; Wang, Nan1,2; Tang, Jilin1 |
| 刊名 | Analyst
 |
| 出版日期 | 2016 |
| 卷号 | 141期号:15页码:4719-4724 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/c6an00745g |
| 通讯作者 | Tang, jilin(jltang@ciac.ac.cn) |
| 英文摘要 | Detection of specific dna sequences is critical in life science. in this study, we investigated the interaction of cobalt oxyhydroxide (coooh) nanoflakes with dna and their fluorescence quenching mechanism of a fam-labeled single-stranded dna (ssdna) probe. ssdna could adsorb on the coooh surface via electrostatic interactions and therefore the fluorescence of fam was quenched. however, upon addition of targets, ssdna was hybridized with target dna and the formed double-stranded dna (dsdna) had much weaker affinity to coooh, resulting in the retaining of fluorescence. based on the affinity difference of coooh nanoflakes to ssdna and dsdna and fluorescence resonance energy transfer based fluorescence quenching, a mix-and-detect method was proposed for homogeneous detection of dna. the proposed method is simple and can be finished in a few minutes with high sensitivity. furthermore, it displays a wide linear range from 1 to 50 nm with a detection limit of 0.5 nm and is capable of detecting dna in real biological samples. |
| WOS关键词 | POLYMERASE-CHAIN-REACTION ; ASCORBIC-ACID ; GOLD NANOPARTICLES ; MOLECULAR BEACON ; DOTS ; BIOMOLECULES ; BIOSENSORS ; NANOPROBE ; EVOLUTION ; PROBES |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000381426100025 |
| 出版者 | ROYAL SOC CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375426 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tang, Jilin |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chang, Yaqing,Zhang, Zhe,Liu, Huiqing,et al. Cobalt oxyhydroxide nanoflake based fluorescence sensing platform for label-free detection of dna[J]. Analyst,2016,141(15):4719-4724. |
| APA | Chang, Yaqing,Zhang, Zhe,Liu, Huiqing,Wang, Nan,&Tang, Jilin.(2016).Cobalt oxyhydroxide nanoflake based fluorescence sensing platform for label-free detection of dna.Analyst,141(15),4719-4724. |
| MLA | Chang, Yaqing,et al."Cobalt oxyhydroxide nanoflake based fluorescence sensing platform for label-free detection of dna".Analyst 141.15(2016):4719-4724. |
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来源：中国科学院大学
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